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https://hdl.handle.net/1822/36602
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Campo DC | Valor | Idioma |
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dc.contributor.author | Praveen, S. | por |
dc.contributor.author | Oliveira, Sara M. | por |
dc.contributor.author | Borges, J. | por |
dc.contributor.author | Mano, J. F. | por |
dc.date.accessioned | 2015-08-07T10:27:32Z | - |
dc.date.available | 2015-08-07T10:27:32Z | - |
dc.date.issued | 2015-01 | - |
dc.date.submitted | 2015-01 | - |
dc.identifier.citation | Praveen S., Oliveira S. M., Borges J., Mano J. F. Assembly of cell-laden hydrogel fiber into non-liquefied and liquefied 3D spiral constructs by perfusion-based layer-by-layer technique, Biofabrication, Vol. 7, Issue 1, pp. 011001-011007, doi:10.1088/1758-5090/7/1/011001, 2015 | por |
dc.identifier.issn | 1758-5082 | por |
dc.identifier.uri | https://hdl.handle.net/1822/36602 | - |
dc.description.abstract | In this work, three-dimensional (3D) self-sustaining, spiral-shaped constructs were produced through a combination of ionotropic gelation, to form cell-encapsulated alginate fibers, and a perfusion-based layer-by-layer (LbL) technique. Single fibers were assembled over cylindrical molds by reeling to form spiral shapes, both having different geometries and sizes. An uninterrupted nanometric multilayer coating produced by a perfusion-based LbL technique, using alginate and chitosan, generated stable 3D spiral-shaped macrostructures by gripping and affixing the threads together without using any crosslinking/binding agent. The chelation process altered the internal microenvironment of the 3D construct from the solid to the liquefied state while preserving the external geometry. L929 cell viability by MTS and dsDNA quantification favor liquefied 3D constructs more than nonliquefied ones. The proposed technique setup helps us to generate complex polyelectrolyte-based 3D constructs for tissue engineering applications and organ printing. | por |
dc.description.sponsorship | The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) for grants SFRH/BPD/48948/2008, SFRH/BD/70107/2010 and project PTDC/CTM-BIO/1814/2012. This work has also received funding from the European Union's 7th framework program (FP7/2007-2013) under grant agreement no REGPOT-CT2012-316331-POLARIS and also from FEDER through the competitive factors operation program (COMPETE). | por |
dc.language.iso | eng | por |
dc.publisher | IOP Publishing | por |
dc.relation | SFRH/BPD/48948/2008 | - |
dc.relation | SFRH/BD/70107/2010 | - |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/127180/PT | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/316331/EU | - |
dc.relation | PTDC/CTM-BIO/1814/2012 | - |
dc.rights | restrictedAccess | por |
dc.subject | Cell-encapsulation | por |
dc.subject | Chelation | por |
dc.subject | Hydrogel fiber | por |
dc.subject | Layer-by-layer | por |
dc.subject | Liquefied core | por |
dc.subject | Switching cell microenviroment | por |
dc.subject | Tissue engineering | por |
dc.subject | tissue engineering Supplementary material for this article is available online | por |
dc.title | Assembly of cell-laden hydrogel fiber into non-liquefied and liquefied 3D spiral constructs by perfusion-based layer-by-layer technique | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://iopscience.iop.org/1758-5090/7/1/011001/article | por |
dc.comments | http://www.3bs.uminho.pt/node/18287 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 011001 | por |
oaire.citationEndPage | 011007 | por |
oaire.citationIssue | 1 | por |
oaire.citationTitle | Biofabrication | por |
oaire.citationVolume | 7 | por |
dc.date.updated | 2015-08-06T13:37:23Z | - |
dc.identifier.doi | 10.1088/1758-5090/7/1/011001 | por |
dc.identifier.pmid | 25562702 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biofabrication | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18287-Manuscript_Biofabrication.pdf Acesso restrito! | 1,34 MB | Adobe PDF | Ver/Abrir |