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https://hdl.handle.net/1822/38508
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Campo DC | Valor | Idioma |
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dc.contributor.author | Amorim, Sara | por |
dc.contributor.author | Martins, A. | por |
dc.contributor.author | Neves, N. M. | por |
dc.contributor.author | Pires, R. A. | por |
dc.contributor.author | Reis, R. L. | por |
dc.date.accessioned | 2015-11-27T16:19:01Z | - |
dc.date.available | 2015-11-27T16:19:01Z | - |
dc.date.issued | 2013-12 | - |
dc.date.submitted | 2013 | - |
dc.identifier.citation | Amorim S., Martins A., Neves N. M., Pires R. A., Reis R. L. Silica nanoparticles as dexamethasone delivery systems able to induce the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells, Journal of Tissue Engineering and Regenerative Medicine, Vol. 7, Issue S1, pp. 6-6, doi:10.1002/term.1822, 2013 | - |
dc.identifier.uri | https://hdl.handle.net/1822/38508 | - |
dc.description.abstract | Bioactive glasses, especially silica-based materials, are reported to pres- ent osteoconductive and osteoinductive properties, fundamental char- acteristics in bone regeneration [1,2]. Additionally, dexamethasone (Dex) is one of the bioactive agents able to induce the osteogenic differ- entiation of mesenchymal stem cells by increasing the alkaline phos- phatase activity, and the expression levels of Osteocalcin and Bone Sialoprotein [3]. Herein, we synthesised silica (SiO2) nanoparticles (that present inherent bioactivity and ability to act as a sustained drug delivery system), and coated their surface using poly-L-lysine (PLL) and hyaluronic acid (HA) using the layer-by-layer processing technique. Further on, we studied the influence of these new SiO2-polyelectrolyte coated nanoparticles as Dex sustained delivery systems. The SiO2 nanoparticles were loaded with Dex (SiO2-Dex) and coated with PLL and HA (SiO2-Dex-PLL-HA). Their Dex release profile was evaluated and a more sustained release was obtained with the SiO2-Dex-PLL-HA. All the particles were cultured with human bone marrow-derived mes- enchymal stem cells (hBMSCs) under osteogenic differentiation culture conditions. hBMSCs adhered, proliferated and differentiated towards the osteogenic lineage in the presence of SiO2 (DLS 174nm), SiO2-Dex (DLS 175nm) and SiO2-Dex-PLL-HA (DLS 679nm). The presence of these materials induced the overexpression of osteogenic transcripts, namely of Osteocalcin, Bone Sialoprotein and Runx2. Scanning Elec- tron Microscopy/Electron Dispersive Spectroscopy analysis demon- strated that hBMSCs synthesised calcium phosphates when cultured with SiO2-Dex and SiO2-Dex-PLL-HA nanoparticles. These results indi- cate the potential use of these SiO2-polyelectrolytes coated nanoparti- cles as dexamethasone delivery systems capable of promoting osteogenic differentiation of hBMSCs. | por |
dc.language.iso | eng | por |
dc.publisher | John Wiley and Sons | por |
dc.rights | openAccess | por |
dc.subject | Delivery systems | por |
dc.subject | Human bone marrow-derived mesenchymal stem cells | por |
dc.subject | Osteogenic differentiation | por |
dc.subject | Silica nanoparticles | por |
dc.title | Silica nanoparticles as dexamethasone delivery systems able to induce the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells | por |
dc.type | conferenceAbstract | - |
dc.peerreviewed | no | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/term.1822/abstract | por |
dc.comments | http://www.3bs.uminho.pt/node/18251 | por |
sdum.publicationstatus | published | por |
oaire.citationConferenceDate | 10 Out. - 12 Out. 2013 | por |
sdum.event.type | conference | por |
oaire.citationStartPage | 6 | por |
oaire.citationEndPage | 6 | por |
oaire.citationIssue | S1 | - |
oaire.citationConferencePlace | Porto, Portugal | por |
oaire.citationTitle | TERM STEM 2013: Nanotechnology as a Tool for improving Tissue Engineering and Regenerative Medicine | por |
oaire.citationVolume | 7 | - |
dc.date.updated | 2015-11-09T17:28:37Z | - |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
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18251-153.pdf | 54 kB | Adobe PDF | Ver/Abrir |