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https://hdl.handle.net/1822/39800
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Campo DC | Valor | Idioma |
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dc.contributor.author | Castro, Vânia I. B. | por |
dc.contributor.author | Carvalho, Carina Martins | por |
dc.contributor.author | Fernandes, Rui D. V. | por |
dc.contributor.author | Pereira-Lima, Sílvia M. M. A. | por |
dc.contributor.author | Castanheira, Elisabete M. S. | por |
dc.contributor.author | Costa, Susana P. G. | por |
dc.date.accessioned | 2016-01-29T18:47:38Z | - |
dc.date.issued | 2016-01 | - |
dc.identifier.citation | V. I. B. Castro, C. M. Carvalho, R. D. V. Fernandes, S. M. M. A. Pereira-Lima, E. M. S. Castanheira, S. P. G. Costa, “Peptaibolin analogues by incorporation of α,α-dialkylglycines: synthesis and study of their membrane permeating ability”, Tetrahedron, 2016, 72, 1024-1030. | por |
dc.identifier.issn | 0040-4020 | por |
dc.identifier.uri | https://hdl.handle.net/1822/39800 | - |
dc.description.abstract | Analogues of Peptaibolin, a peptaibol with antibiotic activity, incorporating α,α-dialkylglycines (Deg, Dpg, and Ac6c) at selected positions were synthesised by MW-SPPS and fully characterized. A control analogue incorporating L-alanine was also prepared. The native peptide and the analogues were studied by fluorescence spectroscopy for their membrane permeating activity. Small unilamellar vesicles (SUVs) of egg phosphatidylcholine/ cholesterol (70:30) containing an encapsulated fluorescence probe (6-carboxyfluorescein) were used as membrane models. The assays of carboxyfluorescein release from SUVs upon peptide addition showed that Peptaibolin-Dpg and Peptaibolin-Ac6c are the most active peptides. These results indicate that the structure of the α,α-dialkylglycines is crucial for the membrane permeating ability of these Peptaibolin analogues. | por |
dc.description.sponsorship | Fundação para a Ciência e Tecnologia (Portugal) | por |
dc.description.sponsorship | The authors acknowledge Fundação para a Ciência e a Tecnologia (Portugal) and FEDERCOMPETE-QREN-EU for financial support through projects PTDC/QUI-BIQ/118389/2010 (FCOMP-01-0124-FEDER-020906), PEst-C/QUI/UI0686/2013 (F-COMP-01-0124-FEDER- 037302), PEst-C/FIS/UI0607/2013 (F-COMP-01-0124-FEDER-022711) and the portuguese NMR network (PTNMR, Bruker Avance III 400-Univ. Minho). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/118389/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/132953/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/132974/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/118389/PT | - |
dc.rights | closedAccess | por |
dc.subject | alpha,alpha-Dialkylglycines | por |
dc.subject | Peptaibolin | por |
dc.subject | Membrane permeating peptides | por |
dc.subject | Model membranes | por |
dc.subject | Fluorescence spectroscopy | por |
dc.title | Peptaibolin analogues by incorporation of α,α-dialkylglycines: synthesis and study of their membrane permeating ability | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.tet.2015.12.079 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1024 | por |
oaire.citationEndPage | 1030 | por |
oaire.citationIssue | 7 | por |
oaire.citationTitle | Tetrahedron | por |
oaire.citationVolume | 72 | por |
dc.identifier.doi | 10.1016/j.tet.2015.12.079 | por |
dc.subject.fos | Ciências Naturais::Ciências Físicas | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Tetrahedron | por |
Aparece nas coleções: | CDF - FAMO - Artigos/Papers (with refereeing) CDQuim - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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manuscript RepositoriUM.pdf Acesso restrito! | 648,44 kB | Adobe PDF | Ver/Abrir |