Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/42341
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Ottoni, C. A. | por |
dc.contributor.author | Simões, Marta Filipa | por |
dc.contributor.author | Fernandes, S. | por |
dc.contributor.author | Santos, Cledir | por |
dc.contributor.author | Lima, Nelson | por |
dc.date.accessioned | 2016-08-03T14:20:44Z | - |
dc.date.available | 2016-08-03T14:20:44Z | - |
dc.date.issued | 2016-08-02 | - |
dc.identifier.citation | Ottoni, C.; Simões, Marta Filipa; Fernandes, S.; Santos, C.; Lima, Nelson, High laccase expression by Trametes versicolor in a simulated textile effluent with different carbon sources and pHs. International Journal of Environmental Research and Public Health, 13(8), 778, 2016 | por |
dc.identifier.issn | 1660-4601 | por |
dc.identifier.uri | https://hdl.handle.net/1822/42341 | - |
dc.description.abstract | Textile effluents are highly polluting and have variable and complex compositions. They can be extremely complex, with high salt concentrations and alkaline pHs. A fixed-bed bioreactor was used in the present study to simulate a textile effluent treatment, where the white-rot fungus, Trametes versicolor, efficiently decolourised the azo dye Reactive Black 5 over 28 days. This occurred under high alkaline conditions, which is unusual, but advantageous, for successful decolourisation processes. Active dye decolourisation was maintained by operation in continuous culture. Colour was eliminated during the course of operation and maximum laccase (Lcc) activity (80.2 UL1) was detected after glycerol addition to the bioreactor. Lcc2 gene expression was evaluated with different carbon sources and pH values based on reverse transcriptase-PCR (polymerase chain reaction). Glycerol was shown to promote the highest lcc2 expression at pH 5.5, followed by sucrose and then glucose. The highest levels of expression occurred between three and four days, which corroborate the maximum Lcc activity observed for sucrose and glycerol on the bioreactor. These results give new insights into the use of T. versicolor in textile dye wastewater treatment with high pHs. | por |
dc.description.sponsorship | Cledir R. Santos thanks to the Universidad de La Frontera (Temuco, Chile) for the partial funding from both the Project DIUFRO DI16-0135 and the Doctoral Programme in Science of Natural Resources. This study was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.rights | openAccess | por |
dc.subject | alkaline conditions | por |
dc.subject | fixed-bed bioreactor | por |
dc.subject | glycerol | por |
dc.subject | Reactive Black 5 | por |
dc.subject | reverse transcriptase-PCR | por |
dc.subject | white-rot fungus | por |
dc.title | High laccase expression by Trametes versicolor in a simulated textile effluent with different carbon sources and pHs | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.mdpi.com/journal/ijerph | por |
dc.comments | CEB40106 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationStartPage | 778 | por |
oaire.citationIssue | 8 | por |
oaire.citationTitle | International Journal of Environmental Research and Public Health | por |
oaire.citationVolume | 13 | por |
dc.date.updated | 2016-08-02T16:59:50Z | - |
dc.identifier.eissn | 1660-4601 | - |
dc.identifier.doi | 10.3390/ijerph13080778 | por |
dc.identifier.pmid | 27490563 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Environmental Research and Public Health | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_40106_1.pdf | 1,4 MB | Adobe PDF | Ver/Abrir |