Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/42726
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Nascimento, A. K. C. | por |
dc.contributor.author | Gonçalves, Clarisse Salomé Nobre | por |
dc.contributor.author | Cavalcanti, M. T. H. | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | Porto, A. L. F. | por |
dc.date.accessioned | 2016-10-04T09:14:02Z | - |
dc.date.available | 2016-10-04T09:14:02Z | - |
dc.date.issued | 2016-12 | - |
dc.identifier.citation | Nascimento, A. K. C.; Nobre, C.; Cavalcanti, M. T. H.; Teixeira, J. A.; Porto, A. L. F., Screening of fungi from the genus Penicillium for production of β- fructofuranosidase and enzymatic synthesis of fructooligosaccharides. Journal of Molecular Catalysis - B Enzymatic, 134, Part A, 70-78, 2016 | por |
dc.identifier.issn | 1381-1177 | por |
dc.identifier.uri | https://hdl.handle.net/1822/42726 | - |
dc.description.abstract | Eight new isolated fungi of the genus Penicillium were evaluated for β- fructofuranosidase (FFase) production. From these, Penicillium citreonigrum was selected for FFase and fructooligosaccharides (FOS) production. The influence of temperature, yeast extract concentration, pH and fermentation time on the FFase activity when using the whole microorganism were evaluated by 24 and 23 designs. The pH was set at 6.5 and no yeast extract was used in the optimization experiments since both shown low significant effects on FFase activity. After optimization, temperature and fermentation time, were set to 25.5 °C and 67.8 h. Under these conditions, the model predicted a FFase production of 301.84 U/mL. The scaled-up process in a 2 L bioreactor enhanced the enzyme productivity up to 1.5 times (6.11 U/mL.h). A concentration of 58.7 g/L of FOS was obtained, where kestose was the main product. Assays performed for enzyme characterization showed that 50 °C and a pH 5.0 are the optimal conditions for FFase activity. FFase showed to be stable at temperatures between 2530 °C and pH 4.0-10.0 and its activity increased in the presence of ions, especially Cu4+. Results obtained in this primary report are a clear indication on the interest of using P. citreonigrum as a source of FFase for further FOS production. | por |
dc.description.sponsorship | CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasília, Brazil) the approved project in network Renorbio − 140366/2014-8 | por |
dc.description.sponsorship | Facepe (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco, Recife, Brasil, AMD-0273-2.00/14) | por |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (Portugal) and POPH/FSE for the PostDoc Grant received (reference SFRH/BPD/87498/2012) | por |
dc.description.sponsorship | Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) | por |
dc.description.sponsorship | Portuguese Foundation for Science and Technology (FCT) under the scope of the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier B.V. | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87498%2F2012/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.rights | openAccess | por |
dc.subject | Penicillium citreonigrum | por |
dc.subject | β-fructofuranosidase | por |
dc.subject | Fructooligosaccharides | por |
dc.subject | beta-Fructofuranosidase | por |
dc.title | Screening of fungi from the genus Penicillium for production of β- fructofuranosidase and enzymatic synthesis of fructooligosaccharides | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.elsevier.com/locate/issn/13811177 | por |
dc.comments | CEB43163 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationStartPage | 70 | por |
oaire.citationEndPage | 78 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationVolume | 134, Part A | por |
dc.date.updated | 2016-10-04T08:30:25Z | - |
dc.identifier.eissn | 1381-1177 | - |
dc.identifier.doi | 10.1016/j.molcatb.2016.09.005 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Molecular Catalysis B: Enzymatic | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_43163_1.pdf | 1,79 MB | Adobe PDF | Ver/Abrir |