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https://hdl.handle.net/1822/44851
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Campo DC | Valor | Idioma |
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dc.contributor.author | Pandey, Kishor | por |
dc.contributor.author | Ferreira, Pedro Eduardo Mendes | por |
dc.contributor.author | Ishikawa, Takeshi | por |
dc.contributor.author | Nagai, Takeharu | por |
dc.contributor.author | Kaneko, Osamu | por |
dc.contributor.author | Yahata, Kazuhide | por |
dc.date.accessioned | 2017-02-27T16:27:32Z | - |
dc.date.available | 2017-02-27T16:27:32Z | - |
dc.date.issued | 2016-03 | - |
dc.date.submitted | 2015-09 | - |
dc.identifier.citation | Pandey, K., Ferreira, P. E., Ishikawa, T., Nagai, T., Kaneko, O., & Yahata, K. (2016). Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor. Scientific reports, 6. doi: 10.1038/srep23454 | por |
dc.identifier.issn | 2045-2322 | por |
dc.identifier.uri | https://hdl.handle.net/1822/44851 | - |
dc.description.abstract | Calcium (Ca2+)-mediated signaling is a conserved mechanism in eukaryotes, including the human malaria parasite, Plasmodium falciparum. Due to its small size (<10?µm) measurement of intracellular Ca2+ in Plasmodium is technically challenging, and thus Ca2+ regulation in this human pathogen is not well understood. Here we analyze Ca2+ homeostasis via a new approach using transgenic P. falciparum expressing the Ca2+ sensor yellow cameleon (YC)-Nano. We found that cytosolic Ca2+ concentration is maintained at low levels only during the intraerythrocytic trophozoite stage (30?nM), and is increased in the other blood stages (>300?nM). We determined that the mammalian SERCA inhibitor thapsigargin and antimalarial dihydroartemisinin did not perturb SERCA activity. The change of the cytosolic Ca2+ level in P. falciparum was additionally detectable by flow cytometry. Thus, we propose that the developed YC-Nano-based system is useful to study Ca2+ signaling in P. falciparum and is applicable for drug screening. | por |
dc.description.sponsorship | We are grateful to Japanese Red Cross Blood Society for providing human RBC and plasma. We also thank Tanaka R, Ogoshi (Sakura) M and Matsumoto N for technical assistance and Templeton TJ for critical reading. This study was conducted at the Joint Usage / Research Center on Tropical Disease, Institute of Tropical Medicine, Nagasaki University, Japan. KP was a Tokyo Biochemical Research Foundation (TBRF, http://www.tokyobrf.or.jp) post-doctoral fellow and PEF was a Japanese Society of Promotion Sciences (JSPS) post-doctoral fellow. This work was supported in part by the TBRF (K.P.), JSPS (P.E.F.), Takeda Science Foundation (K.Y.), Grants-in-Aids for Scientific Research 24590509 (K.Y.), 22390079 (O.K.), and for Scientific Research on Innovative Areas 23117008 (O.K.), MEXT, Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | por |
dc.language.iso | eng | por |
dc.publisher | Nature Publishing Group | por |
dc.rights | openAccess | por |
dc.title | Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.nature.com/articles/srep23454 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 13 | por |
oaire.citationTitle | Scientific Reports | por |
oaire.citationVolume | 6 | por |
dc.date.updated | 2017-02-13T13:00:20Z | - |
dc.identifier.doi | 10.1038/srep23454 | por |
dc.identifier.pmid | 27006284 | por |
dc.subject.fos | Ciências Médicas::Medicina Clínica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Scientific Reports | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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srep23454.pdf | 1,52 MB | Adobe PDF | Ver/Abrir |