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https://hdl.handle.net/1822/46826
Título: | Optimization of lipase production by solid-state fermentation of olive pomace: from flask to laboratory-scale packed-bed bioreactor |
Autor(es): | Oliveira, Felisbela Salgado, José Manuel Abrunhosa, Luís Pérez-Rodríguez, Noelia Domínguez, José M. Venâncio, Armando Belo, Isabel |
Palavras-chave: | Olive pomace Solid-state fermentation A. ibericus Lipase production Packed-bed bioreactor |
Data: | Jul-2017 |
Editora: | Springer Nature |
Revista: | Bioprocess and Biosystems Engineering |
Citação: | Oliveira, Felisbela; Salgado, José Manuel; Abrunhosa, Luís; Pérez-Rodríguez, Noelia; Domínguez, José M.; Venâncio, Armando; Belo, Isabel, Optimization of lipase production by solid-state fermentation of olive pomace: from flask to laboratory-scale packed-bed bioreactor. Bioprocess and Biosystems Engineering, 40(7), 1123-1132, 2017 |
Resumo(s): | Lipases are versatile catalysts with many applications and can be produced by solid-state fermentation (SSF) using agro-industrial wastes. The aim of this work was to maximize the production of Aspergillus ibericus lipase under SSF of olive pomace (OP) and wheat bran (WB), evaluating the effect on lipase production of C/N ratio, lipids, phenols, content of sugars of substrates and nitrogen source addition. Moreover, the implementation of the SSF process in a packed-bed bioreactor and the improvement of lipase extraction conditions were assessed. Low C/N ratios and high content of lipids led to maximum lipase production. Optimum SSF conditions were achieved with a C/N mass ratio of 25.2 and 10.2% (w/w) lipids in substrate, by the mixture of OP:WB (1:1) and supplemented with 1.33% (w/w) (NH4)2SO4. Studies in a packed-bed bioreactor showed that the lower aeration rates tested prevented substrate dehydration, improving lipase production. In this work, the important role of Triton X-100 on lipase extraction from the fermented solid substrate has been shown. A final lipase activity of 223 ± 5 U g1 (dry basis) was obtained after 7 days of fermentation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/46826 |
DOI: | 10.1007/s00449-017-1774-2 |
ISSN: | 1615-7591 |
e-ISSN: | 1615-7605 |
Versão da editora: | http://www.springer.com/chemistry/biotechnology/journal/449 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_46781_1.pdf | 589,79 kB | Adobe PDF | Ver/Abrir |