Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/46862
Título: | Screening of nanocomposite scaffolds arrays using superhydrophobic-wettable micropatterns |
Autor(es): | Leite, Álvaro J. Oliveira, Mariana Braga Caridade, Sofia Glória Ferreira Mano, J. F. |
Palavras-chave: | Array-based screening Combinatorial biomaterials screening Flat platforms High-throughput screening Nanocomposites Nondestructive image analysis |
Data: | Mai-2017 |
Editora: | Wiley-VCH Verlag |
Revista: | Advanced Functional Materials |
Citação: | Leite A. J., Oliveira M.B., Caridade S. G., Mano J. F. Screening of Nanocomposite Scaffolds Arrays Using Superhydrophobic-Wettable Micropatterns, Advanced Functional Materials, Vol. -, Issue -, pp. -, doi:10.1002/adfm.201701219, 2017 |
Resumo(s): | Platforms containing multiple arrays for high-throughput screening are demanded in the development of biomaterial libraries. Here, an array platform for the combinatorial analysis of cellular interactions and 3D porous biomaterials is described. Using a novel method based on computer-aided manufacturing, wettable regions are printed on superhydrophobic surfaces, generating isolated spots. This freestanding benchtop array is used as a tool to deposit naturally derived polymers, chitosan and hyaluronic acid, with bioactive glass nanoparticles (BGNPs) to obtain a scaffold matrix. The effect of fibronectin adsorption on the scaffolds is also tested. The biomimetic nanocomposite scaffolds are shown to be osteoconductive, non-cytotoxic, promote cell adhesion, and regulate osteogenic commitment. The method proves to be suitable for screening of biomaterials in 3D cell cultures as it can recreate a multitude of combinations on a single platform and identify the optimal composition that drives to desired cell responses. The platforms are fully compatible with commercially routine cell culture labware and established characterization methods, allowing for a standard control and easy adaptability to the cell culture environment. This study shows the value of 3D structured array platforms to decode the combinatorial interactions at play in cell microenvironments. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/46862 |
DOI: | 10.1002/adfm.201701219 |
ISSN: | 1616-3028 |
Versão da editora: | http://onlinelibrary.wiley.com/doi/10.1002/adfm.201701219/abstract |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19123-ALeite-AdvancedFunctionalMaterials2017.pdf Acesso restrito! | 3,42 MB | Adobe PDF | Ver/Abrir |