Capture and separation of L-histidine through optimized zinc-decorated magnetic silica spheres

Abstract

Zinc-decorated magnetic silica spheres were developed, optimized and tested for the capture and separation of L-histidine. The magnetic silica spheres were prepared using a simple sol-gel method and show excellent magnetic characteristics, adsorption capacity toward metal ions, and stability in aqueous solution in a wide pH range. The binding capacity of zinc-decorated magnetic silica spheres to histidine proved to be strongly influenced by the morphology, composition and concentration of metal at the surface of the magnetic silica spheres and therefore these parameters should be carefully controlled in order to maximize the performance for protein purification purposes. Optimized zinc-decorated magnetic silica spheres demonstrate a binding capacity to L-histidine of approximately 44 mg.g-1 at the optimum binding pH buffer.