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https://hdl.handle.net/1822/53019
Título: | Harnessing the power of PCR molecular fingerprinting methods and next generation sequencing for understanding structure and function in microbial communities |
Autor(es): | Phadke, Sujal Salvador, Andreia F. Alves, J. I. Bretschger, Orianna Alves, M. M. Pereira, M. A. |
Palavras-chave: | Polymerase chain reaction (PCR) Microbiome Canonical correspondence analysis (CCA)Restriction fragment length polymorphism (T-RFLP) Denaturing gradient gel electrophoresis (DGGE) Next generation sequencing (NGS) Canonical correspondence analysis (CCA) Microbiome Restriction fragment length polymorphism (T-RFLP) |
Data: | 2017 |
Editora: | Springer |
Revista: | Methods in Molecular Biology |
Citação: | Phadke, Sujal; Salvador, Andreia F.; Alves, J. I.; Bretschger, Orianna; Alves, M. Madalena; Pereira, M. Alcina, Harnessing the power of PCR molecular fingerprinting methods and next generation sequencing for understanding structure and function in microbial communities. In Lucília Domingues (Ed.), PCR: Methods and Protocols, Vol. Methods in Molecular Biology 1620, New York: Springer, 2017. ISBN: 978-1-4939-7060-5, 225-248 |
Resumo(s): | Polymerase chain reaction (PCR) is central to methods in molecular ecology. Here, we describe PCR-dependent approaches useful for investigating microbial diversity and its function in various natural, human-associated, and built environment ecosystems. Protocols routinely used for DNA extraction, purification, cloning, and sequencing are included along with various resources for the statistical analysis following gel electrophoresis-based methods (DGGE) and sequencing. We also provide insights into eukaryotic microbiome analysis, sample preservation techniques, PCR troubleshooting, DNA quantification methods, and commonly used ordination techniques. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/53019 |
ISBN: | 978-1-4939-7060-5 |
DOI: | 10.1007/978-1-4939-7060-5_16 |
ISSN: | 1064-3745 |
Versão da editora: | https://link.springer.com/protocol/10.1007/978-1-4939-7060-5_16 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
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document_46795_1.pdf Acesso restrito! | 575,88 kB | Adobe PDF | Ver/Abrir |