Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/53584
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Leitão, M. I. | por |
dc.contributor.author | Raju, B. R. | por |
dc.contributor.author | Sousa, M. J. | por |
dc.contributor.author | Gonçalves, M. Sameiro T. | por |
dc.date.accessioned | 2018-03-27T14:41:48Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | M. I. Leitão, B. R. Raju, M. J. Sousa, M. S. T. Gonçalves, Evaluation of Newly Synthesised Benzo[a]phenoxazinium Chlorides as Fluorescent Probes Using Saccharomyces Cerevisiae as Model Organism. In Proceedings of the 18th Int. Electron. Conf. Synth. Org. Chem., Sciforum Electronic Conference Series, Vol. 18, 2015, b021 | por |
dc.identifier.uri | https://hdl.handle.net/1822/53584 | - |
dc.description.abstract | The potential as fluorescent probes of two recently synthesised water-soluble benzo[a]phenoxazinium salts, mono- or dissubstituted with carboxylic ester groups as terminal of propyl substituents at the amine of position 9, were studied by fluorescence microscopy employing intact, live cells. With the aim of assessing the compounds cellular staining pattern and potential specificity, co-localization experiments were performed using yeast mutants expressing green fluorescent protein (GFP)-tagged proteins that localize to specific intracellular location/organelles. It was found that both compounds display a very high affinity for the vacuolar membrane, being the dissubstituted compound especially important due to its higher fluorescence at lower, less toxic concentrations. | por |
dc.description.sponsorship | PTNMR, Bruker Avance III 400-Univ. Minho, PEst-C/QUI/UI0686/2013 (FCOMP-01-0124-FEDER-037302), (PEst-OE/BIA/UI4050/2014, SFRH/BPD/62881/2009 | por |
dc.language.iso | eng | por |
dc.publisher | Sciforum Electronic Conferences Series | por |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/132953/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/135919/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F62881%2F2009/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | NIR fluorescent probes | por |
dc.subject | Nile Blue derivatives | por |
dc.subject | benzo[a]phenoxazine derivatives | por |
dc.subject | fluorescence microscopy | por |
dc.title | Evaluation of newly synthesised benzo[a]phenoxazinium chlorides as fluorescent probes using Saccharomyces cerevisiae as model organism | por |
dc.type | conferencePaper | por |
dc.peerreviewed | yes | por |
dc.comments | Nesta conferência a comunicação foi publicada em 2014 e o proceeding foi publicado no ano seguinte em 2015. | por |
oaire.citationConferenceDate | 1 - 30 November 2014 | por |
sdum.event.title | 18th Int. Electron. Conf. Synth. Org. Chem. | por |
sdum.event.type | conference | por |
oaire.citationStartPage | b021 | por |
dc.subject.fos | Ciências Naturais::Ciências Químicas | por |
dc.subject.fos | Ciências Naturais::Ciências Biológicas | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
sdum.conferencePublication | Proceedings of the 18th Int. Electron. Conf. Synth. Org. Chem. | por |
Aparece nas coleções: | CDQuim - Comunicações e Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18-ECSOC-3.pdf Acesso restrito! | 240,11 kB | Adobe PDF | Ver/Abrir |