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https://hdl.handle.net/1822/54464
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Campo DC | Valor | Idioma |
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dc.contributor.author | Gonçalves, A. I. | por |
dc.contributor.author | Rodrigues, M. T. | por |
dc.contributor.author | Gomes, Manuela E. | por |
dc.date.accessioned | 2018-04-18T15:24:25Z | - |
dc.date.issued | 2017-09 | - |
dc.date.submitted | 2017-09 | - |
dc.identifier.citation | Gonçalves A. I., Rodrigues M. T., Gomes M. E. Tissue-Engineered Magnetic Cell Sheet Patches For Advanced Strategies in Tendon Regeneration, Acta Biomaterialia, doi:10.1016/j.actbio.2017.09.014, 2017 | por |
dc.identifier.issn | 1742-7061 | por |
dc.identifier.uri | https://hdl.handle.net/1822/54464 | - |
dc.description.abstract | Tendons are powerful 3D biomechanically structures combining a few cells in an intrincated and highly hierarchical niche environment. When tendon homeostasis is compromised, restoration of functionality upon injury is limited and requires alternatives to current augmentation or replacement strategies. Cell sheet technologies are a powerful tool for the fabrication of living extracellular-rich patches towards regeneration of tenotopic defects. Thus, we originally propose the development of magnetically responsive tenogenic patches through magnetic cell sheet (magCSs) technology that enable the remote control upon implantation of the tendon-mimicking constructs. A Tenomodulin positive (TNMD+) subpopulation of cells sorted from a crude population of human adipose stem cells (hASCs) previously identified as being prone to tenogenesis was selected for the magCSs patch construction. We investigated the stability, the cellular co-location of the iron oxide nanoparticles (MNPs), as well as the morphology and mechanical properties of the developed magCSs. Moreover, the expression of tendon markers and collagenous tendon-like matrix were further assessed under the actuation of an external magnetic field. Overall, this study confirms the potential to bioengineer tendon patches using a magnetic cell sheet construction with magnetic responsiveness, good mechanoelastic properties and a tenogenic prone stem cell population envisioning cell-based functional therapies towards tendon regeneration. | por |
dc.description.sponsorship | Authors acknowledge the financial support from FCT/MCTES (Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia, e Ensino Superior) and the Fundo Social Europeu através do Programa Operacional do Capital Humano (FSE/POCH), PD/59/2013, for the PhD grant PD/BD/113802/2015; and FCT for the IF/00685/2012 grant and for the post-doctoral grant SFRH/BPD/111729/2015. This article is a result of the project (Accelerating tissue engineering and personalized medicine discoveries by the integration of key enabling nanotechnologies, marine-derived biomaterials and stem cells), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Authors would like also to acknowledge the HEMS/IBMC/I3S – Institute for Molecular and Cell Biology (IBMC), University of Porto, where the TEM analysis was performed; Dr. Rui M.A. Domingues for the confocal microscopy analysis and Dr. Sofia G. Caridade for the DMA analysis. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | PD/BD/113802/2015 | por |
dc.relation | SFRH/BPD/111729/2015 | por |
dc.rights | restrictedAccess | por |
dc.subject | Adipose stem cell sheets | por |
dc.subject | Magnetic responsiveness | por |
dc.subject | Patch | por |
dc.subject | Tendon Tissue Engineering | por |
dc.subject | tenomodulin | por |
dc.title | Tissue-engineered magnetic cell sheet patches for advanced strategies in tendon regeneration | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S1742706117305779 | por |
dc.comments | http://3bs.uminho.pt/node/19188 | por |
oaire.citationStartPage | 110 | por |
oaire.citationEndPage | 122 | por |
oaire.citationVolume | 63 | por |
dc.date.updated | 2017-09-25T14:37:18Z | - |
dc.identifier.doi | 10.1016/j.actbio.2017.09.014 | por |
dc.identifier.pmid | 28919507 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Acta Biomaterialia | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19188-GoncalvesAI et al_Acta Biomaterialia (2017).pdf Acesso restrito! | 3,31 MB | Adobe PDF | Ver/Abrir |