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https://hdl.handle.net/1822/55077
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Sedano-Núñez, Vicente T. | por |
dc.contributor.author | Boeren, Sjef | por |
dc.contributor.author | Stams, Alfons Johannes Maria | por |
dc.contributor.author | Plugge, Caroline M. | por |
dc.date.accessioned | 2018-06-19T08:42:01Z | - |
dc.date.available | 2018-06-19T08:42:01Z | - |
dc.date.issued | 2018-05 | - |
dc.identifier.citation | Sedano-Núñez, Vicente T.; Boeren, Sjef; Stams, A. J. M.; Plugge, Caroline M., Comparative proteome analysis of propionate degradation by Syntrophobacter fumaroxidans in pure culture and in coculture with methanogens. Environmental Microbiology, 20(5), 1842-1856, 2018 | por |
dc.identifier.issn | 1462-2912 | por |
dc.identifier.uri | https://hdl.handle.net/1822/55077 | - |
dc.description.abstract | Syntrophobacter fumaroxidans is a sulfatereducing bacterium able to grow on propionate axenically or in syntrophic interaction with methanogens or other sulfatereducing bacteria. We performed a proteome analysis of S. fumaroxidans growing with propionate axenically with sulfate or fumarate, and in syntrophy with Methanospirillum hungatei, Methanobacterium formicicum or Desulfovibrio desulfuricans. Special attention was put on the role of hydrogen and formate in interspecies electron transfer (IET) and energy conservation. Formate dehydrogenase Fdh1 and hydrogenase Hox were the main confurcating enzymes used for energy conservation. In the periplasm, Fdh2 and hydrogenase Hyn play an important role in reverse electron transport associated with succinate oxidation. Periplasmic Fdh3 and Fdh5 were involved in IET. The sulfate reduction pathway was poorly regulated and many enzymes associated with sulfate reduction (Sat, HppA, AprAB, DsrAB and DsrC) were abundant even at conditions where sulfate was not present. Proteins similar to heterodisulfide reductases (Hdr) were abundant. Hdr/Flox was detected in all conditions while HdrABC/HdrL was exclusively detected when sulfate was available; these complexes most likely confurcate electrons. Our results suggest that S. fumaroxidans mainly used formate for electron release and that different confurcating mechanisms were used in its sulfidogenic metabolism. This article is protected by copyright | por |
dc.description.sponsorship | This research was supported by the Dutch Technology Foundation (STW) (project 11603), which is part of the Netherlands Organization for Scientific Research (NWO), and which is partly funded by the Ministry of Economic Affairs. Research of AJMS is supported by the European Research Council (ERC grant 323009) and the Gravitation grant (024.002.002) of the Netherlands Ministry of Education, Cultureand Science. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley-Blackwell | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/323009/EU | por |
dc.rights | openAccess | por |
dc.subject | Syntrophy | por |
dc.subject | sulfate-reducing bacteria | por |
dc.subject | propionate oxidation | por |
dc.subject | interspecies electron transfer | por |
dc.subject | reverse electron transport | por |
dc.subject | hydrogenases | por |
dc.subject | formate dehydrogenases | por |
dc.title | Comparative proteome analysis of propionate degradation by Syntrophobacter fumaroxidans in pure culture and in coculture with methanogens | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-2920 | por |
dc.comments | CEB47520 | por |
oaire.citationStartPage | 1842 | por |
oaire.citationEndPage | 1856 | por |
oaire.citationIssue | 5 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationVolume | 20 | por |
dc.date.updated | 2018-04-29T11:45:21Z | - |
dc.identifier.eissn | 1462-2920 | por |
dc.identifier.doi | 10.1111/1462-2920.14119 | por |
dc.identifier.pmid | 29611893 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Environmental Microbiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_47520_1.pdf | 1,86 MB | Adobe PDF | Ver/Abrir |