Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/58194
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Melendez, Matias Eliseo | por |
dc.contributor.author | Silva-Oliveira, Renato José | por |
dc.contributor.author | Silva Almeida Vicente, Anna Luiza | por |
dc.contributor.author | Rebolho Batista Arantes, Lidia Maria | por |
dc.contributor.author | Carolina de Carvalho, Ana | por |
dc.contributor.author | Epstein, Alberto Luis | por |
dc.contributor.author | Reis, R. M. | por |
dc.contributor.author | Carvalho, André Lopes | por |
dc.date.accessioned | 2019-01-15T10:14:06Z | - |
dc.date.available | 2019-01-15T10:14:06Z | - |
dc.date.issued | 2018-06-05 | - |
dc.identifier.citation | Melendez, M. E., Silva-Oliveira, R. J., Vicente, A. L. S. A., Arantes, L. M. R. B., de Carvalho, A. C., Epstein, A. L., ... & Carvalho, A. L. (2018). Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations. Oncotarget, 9(43), 27233. | por |
dc.identifier.uri | https://hdl.handle.net/1822/58194 | - |
dc.description.abstract | Apoptosis induction has emerged as a treatment option for anticancer therapy. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a type II transmembrane protein, is a potent and specific pro-apoptotic protein ligand, which activates the extrinsic apoptosis pathway of the cell death receptors. Here we describe the construction and characterization of a new soluble TRAIL, sfTRAIL, stabilized with the trimerization Foldon domain from the Fibritin protein of the bacteriophage T4. Supernatants of 0.22 μM-filtered supernatants were produced in Vero-transduced cells with HSV1-derived viral amplicon vectors. Experiments were undertaken in two known TRAIL-sensitive (U373 and MDA.MB.231) and two TRAIL-resistant (MCF7 and A549) cell lines, to determine (i) whether the sfTRAIL protein is synthetized and, (ii) whether sfTRAIL could induce receptor-mediated apoptosis. Our results showed that sfTRAIL was able to induce apoptosis at concentrations as low as 1899.29 pg/mL (27.71 pM), independently of caspase-9 activation, and reduction in cell viability at 998.73 fM. | por |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), and to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). MEM, LMRBA and ACL were recipient of FAPESP fellowship. ALC has a CNPq scholarship | por |
dc.language.iso | eng | por |
dc.publisher | Impact Journals | por |
dc.rights | openAccess | por |
dc.subject | TRAIL | por |
dc.subject | Apoptosis | por |
dc.subject | Cancer treatment | por |
dc.subject | Amplicon vectors | por |
dc.title | Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=25519&path[]=79903 | por |
oaire.citationStartPage | 27233 | por |
oaire.citationEndPage | 27241 | por |
oaire.citationIssue | 43 | por |
oaire.citationVolume | 9 | por |
dc.identifier.eissn | 1949-2553 | - |
dc.identifier.doi | 10.18632/oncotarget.25519 | por |
dc.subject.fos | Ciências Médicas::Medicina Básica | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
sdum.journal | Oncotarget | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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melendez 2018.pdf | 2,6 MB | Adobe PDF | Ver/Abrir |