Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/58768
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Franco, Albina Ribeiro | por |
dc.contributor.author | Kimmerling, E. P. | por |
dc.contributor.author | Silva, C. | por |
dc.contributor.author | Rodrigues, F. | por |
dc.contributor.author | Leonor, I. B. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Kaplan, D. L. | por |
dc.date.accessioned | 2019-01-30T12:11:36Z | - |
dc.date.available | 2019-12-01T07:00:16Z | - |
dc.date.issued | 2018-11 | - |
dc.date.submitted | 2018-07 | - |
dc.identifier.citation | Franco A. R., Kimmerling E. P., Silva C., Rodrigues F., Leonor I. B., Reis R. L., Kaplan D. L. Silk‐Based Antimicrobial Polymers as a New Platform to Design Drug‐Free Materials to Impede Microbial Infections, Macromolecular Bioscience, doi:10.1002/mabi.201800262, 2018. | por |
dc.identifier.issn | 1616-5187 | por |
dc.identifier.uri | https://hdl.handle.net/1822/58768 | - |
dc.description.abstract | Surgical site infections (SSI) represent a serious health problem that occur after invasive surgery, thus new antimicrobial biomaterials able to prevent SSI are needed. Silks are natural biopolymers with excellent biocompatibility, low immunogenicity and controllable biodegradability. Spider silkbased materials can be bioengineered and functionalized with specific peptides, such as antimicrobial peptides, creating innovative polymers. Herein, we explored new drugfree multifunctional silk films with antimicrobial properties, specifically tailored to hamper microbial infections. Different spider silk domains derived from the dragline sequence of the spider Nephila clavipes (6mer and 15mer, 27 and 41 kDa proteins, respectively) were fused with the two antimicrobial peptides, Hepcidin (Hep) and Human Neutrophil peptide 1 (HNP1). The selfassembly features of the spider silk domains (sheets) were maintained after functionalization. The bioengineered 6merHNP1 protein demonstrated inhibitory effects against microbial pathogens. Silkbased films with 6merHNP1 and different contents of silk fibroin (SF) significantly reduced bacterial adhesion and biofilm formation, whereas higher bacterial counts were found on the films prepared with 6mer or SF alone. The silkbased films showed no cytotoxic effects on human foreskin fibroblasts. The positive cellular response, together with structural and antimicrobial properties, highlight the potential of these multifunctional silkbased films as new materials for preventing SSI. | eng |
dc.description.sponsorship | The Portuguese Foundation for Science and Technology (FCT) under the project PTDC/BBB-BIO/0827/2012 and by the European Regional Development Fund (FEDER) through the "COMPETE" - Operational Programme for Competitiveness factors (FCOMP-01-0124-FEDER-028120) supported this work. The authors would like to thank Prof. Celia Manaia from the Escola Superior de Biotecnologia (Porto, Portugal) for providing the Pseudomonas sp. bacteria; and Dr. Alberta Faustino from the Hospital de S. Marcos (Braga, Portugal) for providing the other bacterial strains. A. R. Franco thanks FCT through the PostDoctoral scholarship SFRH/BPD/100760/2014. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/125502/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/125502/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F100760%2F2014/PT | - |
dc.rights | openAccess | por |
dc.subject | Antimicrobial peptides | por |
dc.subject | Bacterial adhesion | por |
dc.subject | Biocompatibility | por |
dc.subject | Bioengineered silk proteins | por |
dc.subject | Drug-free polymers | por |
dc.title | Silk‐Based antimicrobial polymers as a new platform to design drug‐free materials to impede microbial infections | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/full/10.1002/mabi.201800262 | por |
dc.comments | http://3bs.uminho.pt/node/19661 | por |
oaire.citationIssue | 12 | por |
oaire.citationVolume | 18 | por |
dc.date.updated | 2019-01-30T09:42:09Z | - |
dc.identifier.eissn | 1616-5195 | por |
dc.identifier.doi | 10.1002/mabi.201800262 | por |
dc.identifier.pmid | 30408337 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.fos | Engenharia e Tecnologia::Biotecnologia Industrial | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Macromolecular Bioscience | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19661-Franco_et_al-2018-Macromolecular_Bioscience.pdf | 3,66 MB | Adobe PDF | Ver/Abrir |