Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61410
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Carvalho, M. R. | por |
dc.contributor.author | Carvalho, C. R. | por |
dc.contributor.author | Maia, F. Raquel | por |
dc.contributor.author | Caballero, David | por |
dc.contributor.author | Kundu, S. C. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Oliveira, Joaquim M. | por |
dc.date.accessioned | 2019-09-17T08:51:21Z | - |
dc.date.available | 2019-09-17T08:51:21Z | - |
dc.date.issued | 2019-09 | - |
dc.date.submitted | 2019-07 | - |
dc.identifier.citation | Carvalho M. R., Carvalho C. R., Maia F. R., Caballero D., Kundu S. C., Reis R. L., Oliveira J. M. Peptide-Modified Dendrimer Nanoparticles for TargetedTherapy of Colorectal Cancer, Advanced Therapeutics, pp. 0-11, doi:10.1002/adtp.201900132, 2019 | por |
dc.identifier.issn | 2366-3987 | por |
dc.identifier.uri | https://hdl.handle.net/1822/61410 | - |
dc.description.abstract | Peptides have recently emerged as a promising class of targeting ligands forspecific drug delivery in cancer treatment, which avoid undesirable side effectsof the systemic administration of chemotherapeutics. Their conjugation withnanoparticles has been demonstrated to improve the functionality of peptidesresulting in a versatile platform for biomedical applications. In this work, thedevelopment of carboxymethylchitosan/poly(amidoamine) (CMCht/PAMAM)dendrimer nanoparticles functionalized with YIGSR laminin receptor bindingpeptide for the active targeting and specific delivery of therapeutic agents intocolorectal cancer cells is described. The successful functionalization isconfirmed by several physico-chemical characterization techniques. Theselectivity of the YIGSR-CMCht/PAMAM dendrimer nanoparticles is firstvalidated in vitro using a micropatterned array of 67 kDa laminin receptor.Next, the specificity of YIGSR-CMCht/PAMAM dendrimers nanoparticlestoward laminin receptor is further confirmed both in 2D and 3D settings usingHCT-116 colorectal cancer cells and L929 fibroblasts in co-culture. Finally,gemcitabine-loaded YIGSR-CMCht/PAMAM dendrimer nanoparticles inducea targeted mortality on HCT-116 cancer cells in a co-culture scenario. Overall,the study shows solid evidence that YIGSR laminin receptor binding peptidecoupled to CMCht/PAMAM dendrimer nanoparticles may be employed as ananticancerous target for the specific and intracellular delivery ofchemotherapeutic agents. | por |
dc.description.sponsorship | This work was financially supported through the project FROnTHERA (NORTE-01-0145-FEDER-000023), Norte Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); M.R.C. for her Ph.D. scholarship NORTE-08-5369-FSE-000044, funded by Programa Operacional Regional do Norte, Fundo Social Europeu, Norte 2020 TERM&SC and EMBO Short-Term Fellowship 7232. J.M.O. for his distinction attributed under the FCT Investigator program (IF/00423/2012 and IF/01285/2015; F.R.M. acknowledges FCT for her work contract under the Transitional Rule DL 57/2016 (CTTI-57/18-I3BS(5)). D.C. acknowledges the financial support from the Portuguese Foundation for Science and Technology (FCT) under the program CEEC Individual 2017 (CEECIND/00352/2017). D.C. and S.C.K. for the Portuguese Foundation for Science and Technology (FCT) under the scope of the project 2MATCH (PTDC/BTMORG/28070/2017) funded by the Programa Operacional Regional do Norte supported by European Regional Development Funds (ERDF). This work is also partially supported by the IET Harvey Engineering Research Award 2018 (ENG ThE CANCER) and the European Union Framework Program for Research and Innovation Horizon 2020 on FoReCaST project under Grant Agreement No. 668983. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley | por |
dc.rights | openAccess | por |
dc.subject | Colorectal cancer | por |
dc.subject | Nanoparticles | por |
dc.subject | Targeting | por |
dc.subject | cancer cells targeting | por |
dc.subject | dendrimers | por |
dc.subject | peptides | por |
dc.title | Peptide-modified dendrimer nanoparticles for targeted therapy of colorectal cancer | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/epdf/10.1002/adtp.201900132 | por |
dc.comments | http://3bs.uminho.pt/node/19913 | por |
oaire.citationIssue | 11 | por |
oaire.citationVolume | 2 | por |
dc.date.updated | 2019-09-16T14:23:05Z | - |
dc.identifier.doi | 10.1002/adtp.201900132 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Advanced Therapeutics | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19913-Carvalho_et_al-2019-Advanced_Therapeutics.pdf | 1,5 MB | Adobe PDF | Ver/Abrir |