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https://hdl.handle.net/1822/61478
Título: | Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife |
Autor(es): | Lyashchenko, K. P. Greenwald, R. Esfandiari, J. Chambers, M. A. Vicente, J. Gortazar, C. Santos, N. Correia-Neves, M Buddle, B. M. Jackson, R. O'Brien, D. J. Schmitt, S. Palmer, M. V. Delahay, R. J. Waters, W. R. |
Palavras-chave: | Animals Animals, Wild Antibodies, Bacterial Antigens, Bacterial Cattle Deer Mustelidae Mycobacterium bovis New Zealand Portugal Serologic Tests Spain Sus scrofa Trichosurus Tuberculosis, Bovine United Kingdom United States Tuberculosis Wildlife Serology |
Data: | 10-Dez-2008 |
Editora: | Elsevier 1 |
Revista: | Veterinary Microbiology |
Citação: | Lyashchenko, K. P., Greenwald, R., Esfandiari, J., et. al.(2008). Animal-side serologic assay for rapid detection of Mycobacterium bovis infection in multiple species of free-ranging wildlife. Veterinary microbiology, 132(3-4), 283-292 |
Resumo(s): | Numerous species of mammals are susceptible to Mycobacterium bovis, the causative agent of bovine tuberculosis (TB). Several wildlife hosts have emerged as reservoirs of M. bovis infection for domestic livestock in different countries. In the present study, blood samples were collected from Eurasian badgers (n=1532), white-tailed deer (n=463), brushtail possums (n=129), and wild boar (n=177) for evaluation of antibody responses to M. bovis infection by a lateral-flow rapid test (RT) and multiantigen print immunoassay (MAPIA). Magnitude of the antibody responses and antigen recognition patterns varied among the animals as determined by MAPIA; however, MPB83 was the most commonly recognized antigen for each host studied. Other seroreactive antigens included ESAT-6, CFP10, and MPB70. The agreement of the RT with culture results varied from 74% for possums to 81% for badgers to 90% for wild boar to 97% for white-tailed deer. Small numbers of wild boar and deer exposed to M. avium infection or paratuberculosis, respectively, did not cross-react in the RT, supporting the high specificity of the assay. In deer, whole blood samples reacted similarly to corresponding serum specimens (97% concordance), demonstrating the potential for field application. As previously demonstrated for badgers and deer, antibody responses to M. bovis infection in wild boar were positively associated with advanced disease. Together, these findings suggest that a rapid TB assay such as the RT may provide a useful screening tool for certain wildlife species that may be implicated in the maintenance and transmission of M. bovis infection to domestic livestock. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/61478 |
DOI: | 10.1016/j.vetmic.2008.05.029 |
ISSN: | 0378-1135 |
e-ISSN: | 1873-2542 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0378113508002046 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Lyashchenko-2008-Animal-side-serologic-assay-for-rap.pdf | 462,25 kB | Adobe PDF | Ver/Abrir |