Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61699
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Luis, Carla | por |
dc.contributor.author | Castaño-Guerrero, Yuselis | por |
dc.contributor.author | Soares, Raquel | por |
dc.contributor.author | Sales, M. G. F. | por |
dc.contributor.author | Fernandes, Rúben | por |
dc.date.accessioned | 2019-10-10T12:55:50Z | - |
dc.date.available | 2019-10-10T12:55:50Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Luis, Carla; Guerrero, Yuselis; Castaño-Guerrero, Yuselis; Soares, Raquel; Sales, M. G. F.; Fernandes, Rúben, Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line. Methods and Protocols, 2(2), 29, 2019 | por |
dc.identifier.uri | https://hdl.handle.net/1822/61699 | - |
dc.description.abstract | Doxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in engaging MTT assay using DOXO that exhibits a strong red coloration and fluorescence, and so it is assumed that DOXO may interfere with commonly used colorimetric assays such as MTT. The interference of DOXO in the MTT determination was evaluated in a Breast Cancer cell line Michigan Cancer Foundation-7 (MCF-7). The interference was evaluated by means of spectroscopic methods in particular spectrophometry and fluorescence spectroscopy of MTT and DOXO. We postulate that the medium and the MTT reagent itself can interfere on the metabolic activity method, so in order to achieve better results, DMEM was replaced by a neutral buffer like Phosphate-buffered saline (PBS). This protocol may be extremely useful in future studies involving DOXO. | por |
dc.description.sponsorship | Rúben Fernandes acknowledges, on the behalf of the authors, the funding entities. Carla Luís was supported by a scholarship with the reference SAICT2016/FEDER/BIO4DIA/BTI under the supervision of Dr. Rúben Fernandes. This work was also partially supported by the grant “IBEROS—Instituto de Bioengenharia em Rede para o envelhecimento saudável”, 0245-IBEROS-1-E, cofinanced by the program INTERREG V-A POCTEP through FEDER to BIL/IBEROS-BioMark/03 developed under the supervision of Dr. Goreti Sales. This work was also supported by FCT - Fundação para a Ciência e Tecnologia (REF UID/BIM/04293/2013), by grants NORTE-01-0145-FEDER-000012, SAICT-POL/24325/2016, and SAICT-POL/24358/2016 to Drs. Goreti Sales, Raquel Soares, and Rúben Fernandes. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147342/PT | por |
dc.relation | SAICT-POL/24358/2016 | - |
dc.relation | SAICT-POL/24325/2016 | - |
dc.rights | openAccess | por |
dc.subject | MTT | por |
dc.subject | Doxorubicin | por |
dc.subject | Chemotherapy | por |
dc.subject | Breast cancer | por |
dc.subject | MCF-7 | por |
dc.title | Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/journal/mps | por |
dc.comments | CEB52045 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 5 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 2 | por |
dc.date.updated | 2019-10-07T11:10:29Z | - |
dc.identifier.eissn | 2409-9279 | por |
dc.identifier.doi | 10.3390/mps2020029 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Methods and Protocols | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_52045_1.pdf | 562,26 kB | Adobe PDF | Ver/Abrir |