Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/64979
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Gonçalves, Bruna | por |
dc.contributor.author | Bernardo, Ruben | por |
dc.contributor.author | Wang, Can | por |
dc.contributor.author | Schroeder, Markus | por |
dc.contributor.author | Pedro, Nuno A. | por |
dc.contributor.author | Butler, Geraldine | por |
dc.contributor.author | Azeredo, Joana | por |
dc.contributor.author | Henriques, Mariana | por |
dc.contributor.author | Mira, Nuno Pereira | por |
dc.contributor.author | Silva, Sónia Carina | por |
dc.date.accessioned | 2020-04-21T13:16:57Z | - |
dc.date.available | 2020-04-21T13:16:57Z | - |
dc.date.issued | 2020-04 | - |
dc.identifier.citation | Gonçalves, Bruna; Bernardo, Ruben; Wang, Can; Schroeder, Markus; Pedro, Nuno A.; Butler, Geraldine; Azeredo, Joana; Henriques, Mariana; Mira, Nuno Pereira; Silva, Sónia Carina, Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis. International Journal of Medical Microbiology, 310(3), 151414, 2020 | por |
dc.identifier.issn | 1438-4221 | por |
dc.identifier.uri | https://hdl.handle.net/1822/64979 | - |
dc.description | Supplementary material related to this article can be found, in the online version, at doi: https://doi.org/10.1016/j.ijmm.2020.151414. | por |
dc.description.abstract | Vulvovaginal candidiasis (VVC) caused by Candida albicans is a common disease worldwide. A very important C. albicans virulence factor is its ability to form biofilms on epithelium and/or on intrauterine devices promoting VVC. It has been shown that VVC has a hormonal dependency and that progesterone affects virulence traits of C. albicans cells. To understand how the acidic environment (pH 4) and progesterone (either alone and in combination) modulate C. albicans response during formation of biofilm, a transcriptomic analysis was performed together with characterization of the biofilm properties. Compared to planktonic cells, acidic biofilm-cells exhibited major changes in their transcriptome, including modifications in the expression of 286 genes that were not previously associated with biofilm formation in C. albicans. The vast majority of the genes up-regulated in the acidic biofilm cells (including those uniquely identified in our study) are known targets of Sfl1, and consistently, Sfl1 deletion is herein shown to impair the formation of acidic biofilms (pH4). Under the acidic conditions used, the presence of progesterone reduced C. albicans biofilm biomass and structural cohesion. Transcriptomic analysis of biofilms developed in the presence of progesterone led to the identification of 65 down-regulated genes including, among others, the regulator Tec1 and several of its target genes, suggesting that the function of this transcription factor is inhibited by the presence of the hormone. Additionally, progesterone reduced the susceptibility of biofilm cells to fluconazole, consistent with an up-regulation of efflux pumps. Overall, the results of this study show that progesterone modulates C. albicans biofilm formation and genomic expression under acidic conditions, which may have implications for C. albicans pathogenicity in the vaginal environment. | por |
dc.description.sponsorship | This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145- FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Funding received by iBB–Institute for Bioengineering and Biosciences from FCT (UID/BIO/04565/2013), from Programa Operacional Regional de Lisboa 2020 Project No. 007317 is also acknowledged. Support from FCT to NAP through the doctoral program Applied and Environmental Microbiology (PD/BD/143026/2018) is also acknowledged. B. Gonçalves is recipient of a PhD grant supported by FCT (SFRH/BD/111645/2015). G. Butler was supported by Science Foundation Ireland (12/IA/1343). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | UID/BIO/04469/2019 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147338/PT | por |
dc.relation | PD/BD/143026/2018 | por |
dc.relation | SFRH/BD/111645/2015 | por |
dc.rights | openAccess | por |
dc.subject | Vulvovaginal candidiasis | por |
dc.subject | vaginal environment | por |
dc.subject | biofilm regulators | por |
dc.subject | expression profile | por |
dc.title | Effect of progesterone on Candida albicans biofilm formation under acidic conditions: a transcriptomic analysis | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/international-journal-of-medical-microbiology/ | por |
dc.comments | CEB53532 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 310 | por |
dc.date.updated | 2020-03-28T13:35:06Z | - |
dc.identifier.eissn | 1618-0607 | por |
dc.identifier.doi | 10.1016/j.ijmm.2020.151414 | por |
dc.identifier.pmid | 32173268 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Medical Microbiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_53532_1.pdf | 3,16 MB | Adobe PDF | Ver/Abrir |