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https://hdl.handle.net/1822/65111
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Breia, Richard Maykel Gonçalves | por |
dc.contributor.author | Mósca, Andreia F. | por |
dc.contributor.author | Conde, Artur | por |
dc.contributor.author | Correia, Sofia | por |
dc.contributor.author | Conde, Carlos | por |
dc.contributor.author | Noronha, Henrique | por |
dc.contributor.author | Soveral, Graça | por |
dc.contributor.author | Gonçalves, Berta | por |
dc.contributor.author | Gerós, H. | por |
dc.date.accessioned | 2020-04-28T15:29:12Z | - |
dc.date.available | 2020-04-28T15:29:12Z | - |
dc.date.issued | 2020-04-24 | - |
dc.identifier.citation | Breia, Richard; Mósca, Andreia F.; Conde, Artur; Correia, Sofia; Conde, Carlos; Noronha, Henrique; Soveral, Graça; Gonçalves, Berta; Gerós, Hernâni, Sweet Cherry (Prunus avium L.) PaPIP1;4 Is a functional aquaporin upregulated by pre-harvest calcium treatments that prevent cracking. International Journal of Molecular Sciences, 21(8), 3017, 2020 | por |
dc.identifier.isbn | 1424-6783 | - |
dc.identifier.issn | 1661-6596 | por |
dc.identifier.uri | https://hdl.handle.net/1822/65111 | - |
dc.description.abstract | The involvement of aquaporins in rain-induced sweet cherry (Prunus avium L.) fruit cracking is an important research topic with potential agricultural applications. In the present study, we performed the functional characterization of PaPIP1;4, the most expressed aquaporin in sweet cherry fruit. Field experiments focused on the pre-harvest exogenous application to sweet cherry trees, cultivar Skeena, with a solution of 0.5% CaCl2, which is the most common treatment to prevent cracking. Results show that PaPIP1;4 was mostly expressed in the fruit peduncle, but its steady-state transcript levels were higher in fruits from CaCl2-treated plants than in controls. The transient expression of PaPIP1;4-GFP in tobacco epidermal cells and the overexpression of PaPIP1;4 in YSH1172 yeast mutation showed that PaPIP1;4 is a plasma membrane protein able to transport water and hydrogen peroxide. In this study, we characterized for the first time a plasma membrane sweet cherry aquaporin able to transport water and H2O2 that is upregulated by the pre-harvest exogenous application of CaCl2 supplements. | por |
dc.description.sponsorship | This work was supported by the “Contrato-Programa” UIDB/04050/2020 and UIDB/04033/2020 funded by national funds through the FCT I.P. The work was also supported by FCT and European Funds (FEDER/POCI/COMPETE2020) through the research projects MitiVineDrought (PTDC/BIA-FBT/30341/2017 and POCI-01-0145-FEDER-030341), BerryPlastid (PTDC/BIA-FBT/28165/2017 and POCI-01-0145-FEDER-028165) and CherryCrackLess (PTDC/AGR-PRO/7028/2014). R.B. was supported with a PhD student grant (PD/BD/113616/2015) under the Agrichains Doctoral Program (PD/00122/2012) funded by FCT. H.N. was supported by an FCT postdoctoral grant (SFRH/BPD/115518/2016) and A.C. was supported by a contract in the MitiVineDrought project. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.relation | UIDB/04033/2020 | por |
dc.relation | PTDC/BIA-FBT/30341/2017 | por |
dc.relation | PTDC/BIA-FBT/28165/2017 | por |
dc.relation | PTDC/AGR-PRO/7028/2014 | por |
dc.relation | SFRH/BPD/115518/2016 | por |
dc.relation | PD/00122/2012 | por |
dc.relation | PD/BD/113616/2015 | por |
dc.rights | openAccess | por |
dc.subject | aquaporins | por |
dc.subject | PaPIP1 | por |
dc.subject | 4 | por |
dc.subject | calcium application | por |
dc.subject | fruit-cracking | por |
dc.subject | sweet-cherry | por |
dc.subject | PaPIP1;4 | por |
dc.title | Sweet Cherry (Prunus avium L.) PaPIP1;4 is a functional aquaporin upregulated by pre-harvest calcium treatments that prevent cracking | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/21/8/3017 | por |
dc.comments | CEB53648 | por |
oaire.citationIssue | 8 | por |
oaire.citationVolume | 21 | por |
dc.date.updated | 2020-04-26T11:03:06Z | - |
dc.identifier.eissn | 1661-6596 | por |
dc.identifier.doi | 10.3390/ijms21083017 | por |
dc.identifier.pmid | 32344729 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Molecular Sciences | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53648_1.pdf | 3,26 MB | Adobe PDF | Ver/Abrir |