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https://hdl.handle.net/1822/65358
Título: | Paper-based (bio)sensor for label-free detection of 3-nitrotyrosine in human urine samples using molecular imprinted polymer |
Autor(es): | Martins, Gabriela Marques, Ana C. Fortunato, Elvira Sales, M. G. F. |
Palavras-chave: | 3-nitrotyrosine Molecular imprinted polymer Electrochemical (bio)sensor Urine biomarker Carbon-printed electrode |
Data: | Jun-2020 |
Editora: | Elsevier BV |
Revista: | Sensing and Bio-Sensing Research |
Citação: | Martins, Gabriela; Marques, Ana C.; Fortunato, Elvira; Sales, M. G. F., Paper-based (bio)sensor for label-free detection of 3-nitrotyrosine in human urine samples using molecular imprinted polymer. Sensing and Bio-Sensing Research, 28(100333), 2020 |
Resumo(s): | Over the last years, paper technology has been widely spread as a more affordable, sustainable and reliable support material to be incorporated in the design of point-of-care (POC) diagnostic devices. However, the single work employing a paper-based device for 3-nitrotyrosine (3-NT), a relevant biomarker for oxidative stress (OS) that is a major origin for many diseases, is incapable of reading successfully complex samples because every species that oxidizes before ~0.75?V will also contribute to the final response. Thus, the introduction of a selective element was made into this set-up by including a molecularly-imprinted polymer (MIP) tailored in-situ. Herein, a novel MIP for 3-NT was assembled directly on a paper platform, made conductive with carbon ink and suitable for an electrochemical transduction. The biomimetic material was produced by electropolymerization of phenol after optimizing several experimental parameters, such a scan-rate, number of cycles, range of potential applied, monomer and template concentrations. Under optimal conditions, the label-free sensor was able to respond to 3-NT from 500?nM to 1?mM, yielding a limit of detection of 22.3?nM. Finally, the applicability of the (bio)sensor was tested by performing calibration assays in human urine samples and a good performance was obtained in terms of sensitivity, selectivity and reproducibility. Overall, the attributes of the herein described sensing approach can be compared to a very limited number of other electrochemical devices, that are still using a conventional three electrode system, making this paper-sustained device the first electrochemical (bio)sensor with potential to become a portable and low-cost diagnostic tool for 3-NT. In general, the incorporation of molecular imprinting technology coupled to electrochemical transduction enabled the fabrication of suitable smart sensors for wide screening approaches. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/65358 |
DOI: | 10.1016/j.sbsr.2020.100333 |
e-ISSN: | 2214-1804 |
Versão da editora: | https://www.journals.elsevier.com/sensing-and-bio-sensing-research |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53546_1.pdf | 1,66 MB | Adobe PDF | Ver/Abrir |