Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/67240
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | de Carvalho, Ana Carolina | por |
dc.contributor.author | de Mattos Pereira, Leandro | por |
dc.contributor.author | Datorre, José Guilherme | por |
dc.contributor.author | Dos Santos, Wellington | por |
dc.contributor.author | Berardinelli, Gustavo Noriz | por |
dc.contributor.author | Matsushita, Marcus de Medeiros | por |
dc.contributor.author | Oliveira, Marco Antonio | por |
dc.contributor.author | Durães, Ronilson Oliveira | por |
dc.contributor.author | Guimarães, Denise Peixoto | por |
dc.contributor.author | Reis, R. M. | por |
dc.date.accessioned | 2020-10-02T10:07:40Z | - |
dc.date.available | 2020-10-02T10:07:40Z | - |
dc.date.issued | 2019-08 | - |
dc.identifier.citation | de Carvalho, A. C., Pereira, L. D. M., Datorre, J. G., et. al.(2019). Microbiota profile and impact of Fusobacterium nucleatum in colorectal cancer patients of Barretos Cancer Hospital. Frontiers in oncology, 9, | por |
dc.identifier.issn | 2234-943X | por |
dc.identifier.uri | https://hdl.handle.net/1822/67240 | - |
dc.description.abstract | Microbial diversity has been pointed out as a major factor in the development and progression of colorectal cancer (CRC). We sought to explore the richness and abundance of the microbial community of a series of colorectal tumor samples treated at Barretos Cancer Hospital, Brazil, through 16S rRNA sequencing. The presence and the impact of Fusobacterium nucleatum (Fn) DNA in CRC prognosis was further evaluated by qPCR in a series of 152 CRC cases. An enrichment for potentially oncogenic bacteria in CRC was observed, with Fusobacterium being the most abundant genus in the tumor tissue. In the validation dataset, Fn was detected in 35/152 (23.0%) of fresh-frozen tumor samples and in 6/57 (10.5%) of paired normal adjacent tissue, with higher levels in the tumor (p = 0.0033). Fn DNA in the tumor tissue was significantly associated with proximal tumors (p = 0.001), higher depth of invasion (p = 0.014), higher clinical stages (p = 0.033), poor differentiation (p = 0.011), MSI-positive status (p < 0.0001), BRAF mutated tumors (p < 0.0001), and the loss of expression of mismatch-repair proteins MLH1 (p < 0.0001), MSH2 (p = 0.003), and PMS2 (p < 0.0001). Moreover, the presence of Fn DNA in CRC tissue was also associated with a worse patient cancer-specific survival (69.9 vs. 82.2% in 5 years; p = 0.028) and overall survival (63.5 vs. 76.5%; p = 0.037). Here we report, for the first time, the association of F. nucleatum presence with important clinical and molecular features in a Brazilian cohort of CRC patients. Tumor detection and classification based on the gut microbiome might provide a promising approach to improve the prediction of patient outcome. | por |
dc.description.sponsorship | Barretos Cancer Hospital, Public Ministry of Labor Campinas (Research, Prevention, and Education of Occupational Cancer), and Coordination for the Improvement of Higher Education Personnel (Capes, Brazil) | por |
dc.language.iso | eng | por |
dc.publisher | Frontiers Media | por |
dc.rights | openAccess | por |
dc.subject | Colorectal cancer | por |
dc.subject | Microbiota | por |
dc.subject | Fusobacterium nucleatum | por |
dc.subject | MSI | por |
dc.subject | Patient survival | por |
dc.title | Microbiota profile and impact of fusobacterium nucleatum in colorectal cancer patients of Barretos cancer hospital | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fonc.2019.00813/full | por |
oaire.citationIssue | AUG | por |
oaire.citationVolume | 9 | por |
dc.identifier.eissn | 2234-943X | - |
dc.identifier.doi | 10.3389/fonc.2019.00813 | por |
dc.subject.fos | Ciências Médicas::Medicina Básica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Frontiers in Oncology | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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De-carvalho-2019-Microbiota-profile-and-impact-of-fu.pdf | 823,54 kB | Adobe PDF | Ver/Abrir |