Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/67979
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Oliveira, D. M. L. | por |
dc.contributor.author | Rezende, P. S. | por |
dc.contributor.author | Barbosa, T. C. | por |
dc.contributor.author | Nalone, L. A. | por |
dc.contributor.author | Bani, C. | por |
dc.contributor.author | Tavares, D. S. | por |
dc.contributor.author | Silva, C. F. da | por |
dc.contributor.author | Chaud, M. V. | por |
dc.contributor.author | Padilha, F. | por |
dc.contributor.author | Cano, A. | por |
dc.contributor.author | Albuquerque Júnior, R. L. C. de | por |
dc.contributor.author | Souto, Eliana B. | por |
dc.contributor.author | Severino, P. | por |
dc.date.accessioned | 2020-11-02T17:37:10Z | - |
dc.date.available | 2020-11-02T17:37:10Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Oliveira, D. M. L.; Rezende, P. S.; Barbosa, T. C.; Nalone, L. A.; Bani, C.; Tavares, D. S.; Silva, C. F. da; Chaud, M. V.; Padilha, F.; Cano, A.; Albuquerque Júnior, R. L. C. de; Souto, Eliana; Severino, P., Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair. International Journal of Pharmaceutics, 591(120001), 2020 | por |
dc.identifier.issn | 0378-5173 | por |
dc.identifier.uri | https://hdl.handle.net/1822/67979 | - |
dc.description.abstract | The aim of this study was to develop and characterize a double layer biomembrane for dual drug delivery to be used for the treatment of wounds. The membrane was composed of chitosan, hydroxypropyl methylcellulose and lidocaine chloride (anesthetic drug) in the first layer, and of sodium alginate-polymyxin B sulphate (antibiotic) nanoparticles as the second layer. A product with excellent thickness (0.01-0.02 mm), adequate mechanical properties with respect to elasticity, stiffness, tension, and compatible pH for lesion application has been successfully obtained. The incorporation of the drugs was confirmed analysing the membrane cross-sections by scanning electron microscopy. A strong interaction between the drugs and the functional groups of respective polymers was confirmed by Fourier-Transform Infrared Spectroscopy, thermal analysis and X-ray diffraction. Microbiological assays showed a high antimicrobial activity when polymyxin B was present to act against the Staphylococcus aureus and Pseudomonas aeruginosa strains. Low cytotoxicity observed in a cell viability colorimetric assay and SEM analysis suggest biocompatibility between the developed biomembrane and the cell culture. The in vivo assay allowed visualizing the healing potential by calculating the wound retraction index and by histological analysis. Our results confirm the effectiveness of the developed innovative biomaterial for tissue repair and regeneration in an animal model. | por |
dc.description.sponsorship | The authors acknowledge the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/Brazil), Fundação de Amparo a Pesquisa do Estado de Sergipe (FAPITEC, MS/CNPq/FAPITEC/SE/SES N◦06/2018) e do Conselho Nacional de Pesquisas (CNPq, Apoio a Projetos de Pesquisa/MCTI/CNPQ/Universal 14/2014) for supporting funds. EBS acknowledges the Portuguese Science and Technology Foundation (FCT) for the funded projects M-ERA-NET/0004/2015 (PAIRED) and UIDB/04469/2020 (strategic fund) financed from national funds, and co-financed Education (FCT/MEC) from national funds and FEDER, under the Partnership Agreement PT2020. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | UIDB/04469/2020 | por |
dc.rights | openAccess | por |
dc.subject | double membrane | por |
dc.subject | polymyxin B sulphate | por |
dc.subject | lidocaine hydrochloride | por |
dc.subject | solid lipid nanoparticles | por |
dc.subject | burst release | por |
dc.subject | controlled release | por |
dc.title | Double membrane based on lidocaine-coated polymyxin-alginate nanoparticles for wound healing: in vitro characterization and in vivo tissue repair | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.elsevier.com/locate/issn/03785173 | por |
dc.comments | CEB53951 | por |
oaire.citationVolume | 591 | por |
dc.date.updated | 2020-10-31T11:45:15Z | - |
dc.identifier.doi | 10.1016/j.ijpharm.2020.120001 | por |
dc.identifier.pmid | 33141086 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Pharmaceutics | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53951_1.pdf | 15,22 MB | Adobe PDF | Ver/Abrir |