Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/73041
Título: | Elastin-based nanoparticles for delivery of bone morphogenetic proteins |
Autor(es): | Machado, Raul Bessa, Paulo C. Reis, R. L. Rodriguez-Cabello, José Carlos Casal, Margarida |
Palavras-chave: | BMP-2 Elastin-like polymer Encapsulation Nanoparticles Poly(VPAVG) Self-assembly |
Data: | 15-Ago-2012 |
Revista: | Methods in Molecular Biology |
Citação: | Machado, R., Bessa, P. C., Reis, R. L., Rodriguez-Cabello, J. C., & Casal, M. (2012). Elastin-based nanoparticles for delivery of bone morphogenetic proteins. In Nanoparticles in Biology and Medicine (pp. 353-363). Humana Press, Totowa, NJ. |
Resumo(s): | Biocompatibility, stability, and efficiency remain among the major goals in the development of new drug delivery systems. Herein we describe a facile method for encapsulation of Bone Morphogenetic Protein-2 (BMP-2) at high concentrations and in mild conditions by exploiting the thermo-responsiveness of an elastin-like polymer. Chemically synthesized poly(VPAVG) demonstrates excellent biocompatibility, stability at room temperature, and allows the use of mild conditions and harmless solvents, such as water, for its production. Here, we describe how to use a recombinantly produced poly(VPAVG) polymer to entrap BMP-2 with a high encapsulation efficiency and the methods for in vitro bioactivity assays. © 2012 Springer Science+Business Media, LLC. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/73041 |
ISBN: | 9781617799525 |
DOI: | 10.1007/978-1-61779-953-2_29 |
ISSN: | 1064-3745 2279-1448 |
Versão da editora: | https://link.springer.com/protocol/10.1007/978-1-61779-953-2_29 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CBMA - Livros e Capítulos de Livros/Books and Books Chapters |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Chapter 29.pdf Acesso restrito! | 362,94 kB | Adobe PDF | Ver/Abrir |