Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/73411
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Oliveira, Ana Isabel | por |
dc.contributor.author | Pinho, Cláudia | por |
dc.contributor.author | Fonte, Pedro | por |
dc.contributor.author | Sarmento, Bruno | por |
dc.contributor.author | Dias, Alberto Carlos Pires | por |
dc.date.accessioned | 2021-06-16T09:47:54Z | - |
dc.date.available | 2021-06-16T09:47:54Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Oliveira, A. I., Pinho, C., Fonte, P., Sarmento, B., & Dias, A. C. P. (2018). Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum. International Journal of Biological Macromolecules, 110, 185-196. doi: https://doi.org/10.1016/j.ijbiomac.2017.10.103 | por |
dc.identifier.issn | 0141-8130 | por |
dc.identifier.uri | https://hdl.handle.net/1822/73411 | - |
dc.description.abstract | Quercetin and biapigenin are antioxidant and neuroprotective compounds present in Hypericum perforatum, with potential application in neurodegenerative diseases. Quercetin has shown to have excellent antioxidant activities. Biapigenin possesses a distinctive mechanism of action, preventing the onset of calcium deregulation and mitochondrial dysfunction. The main aim of this study was to establish a new delivery system encapsulating H. perforatum neuroprotective fraction, isolated from it,containing quercetin and biapigenin into poly(Ɛ-caprolactone) (PCL) nanoparticles. It also aimed to study its hepatoprotective potential. Different formulation parameters were optimized, resulting in a stable formulation of polymer:compounds ratio 1:0.1 with a mean particle size of 185nm, zeta potential around -20mV and association efficiency close to 100%. Compounds in vitro release, under physiological conditions, showed an initial burst followed by a sustained release. The antioxidant functional properties of these compounds were not altered by encapsulation. PCL-loaded nanoparticles protected HepG2 cells from intrinsic compound toxicity at high concentrations. Depending on the incubation regimen, quercetin-biapigenin PCL-loaded nanoparticles or free compounds were more effective in protecting HepG2 cells against tert-butylhydroperoxide-induced toxicity. This is the first report of the encapsulation of a quercetin-biapigenin mixture in a polymeric matrix, specifically, in PCL, with synergic anti-oxidant and hepatoprotective effects. | por |
dc.description.sponsorship | The authors would like to thank Rui Fernandes, from I3S and IBMC, for TEM analysis. This work was supported by Fundação para a Ciência e Tecnologia (FCT, Portugal), projects PTDC/AGRALI/105169/2008, PEst-OE/AGR/UI4033/2014. Ana Isabel Oliveira was supported by Escola Superior de Tecnologia da Saúde do Porto and Instituto Politécnico do Porto (Programa de Formação Avançada de Docentes). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/105169/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/135895/PT | por |
dc.rights | openAccess | por |
dc.subject | Hep G2 Cells | por |
dc.subject | Humans | por |
dc.subject | Hypericum perforatum | por |
dc.subject | Antioxidants | por |
dc.subject | Drug Carriers | por |
dc.subject | Nanoparticles | por |
dc.subject | Neuroprotective Agents | por |
dc.subject | Polyesters | por |
dc.subject | Quercetin | por |
dc.subject | Biapigenin | por |
dc.subject | Hepatoprotective | por |
dc.subject | In vitro release | por |
dc.subject | Anti-oxidant | por |
dc.subject | Anti-oxidant | por |
dc.title | Development, characterization, antioxidant and hepatoprotective properties of poly(Ɛ-caprolactone) nanoparticles loaded with a neuroprotective fraction of Hypericum perforatum | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
oaire.citationStartPage | 185 | por |
oaire.citationEndPage | 196 | por |
oaire.citationVolume | 110 | por |
dc.identifier.doi | 10.1016/j.ijbiomac.2017.10.103 | por |
dc.identifier.pmid | 29080823 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Biological Macromolecules | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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(2018) oliveira.pdf | 2,25 MB | Adobe PDF | Ver/Abrir |