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https://hdl.handle.net/1822/74300
Título: | Sustained release of a Streptococcus pneumoniae endolysin from liposomes for potential otitis media treatment |
Autor(es): | Silva, Maria Daniela Ferreira Paris, Juan L. Gama, F. M. Silva, Bruno F. B. Sillankorva, Sanna |
Palavras-chave: | Endolysin MSlys Streptococcus pneumoniae Antibacterial Liposome Drug delivery |
Data: | 24-Jun-2021 |
Editora: | American Chemical Society |
Revista: | ACS Infectious Diseases |
Citação: | Silva, Maria Daniela; Paris, Juan L.; Gama, F. M.; Silva, Bruno F. B.; Sillankorva, Sanna, Sustained release of a Streptococcus pneumoniae endolysin from liposomes for potential otitis media treatment. ACS Infectious Diseases, 7(8), 2127-2137, 2021 |
Resumo(s): | Local delivery of antimicrobials for otitis media treatment would maximize therapeutic efficacy while minimizing side effects. However, drug transport across the tympanic membrane in the absence of a delivery system is challenging. In this study, the MSlys endolysin was encapsulated in deformable liposomes for a targeted treatment of S. pneumoniae, one of the most important causative agents of otitis media. MSlys was successfully encapsulated in liposomes composed of l-alpha-lecithin and sodium cholate (5:1) or l-alpha-lecithin and PEG2000 PE (10:1), with encapsulation efficiencies of about 35\%. The PEGylated and sodium cholate liposomes showed, respectively, mean hydrodynamic diameters of 85 and 115 nm and polydispersity indices of 0.32 and 0.42, both being stable after storage at 4 °C for at least one year. Both liposomal formulations showed a sustained release of MSlys over 7 days. Cytotoxicity studies against fibroblast and keratinocyte cell lines revealed the biocompatible nature of both MSlys and MSlys-loaded liposomes. Additionally, the encapsulated MSlys showed prompt antipneumococcal activity against planktonic and biofilm S. pneumoniae, thus holding great potential for transtympanic treatment against S. pneumoniae otitis media. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/74300 |
DOI: | 10.1021/acsinfecdis.1c00108 |
ISSN: | 2373-8227 |
e-ISSN: | 2373-8227 |
Versão da editora: | https://pubs.acs.org/journal/aidcbc |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54495_1.pdf Acesso restrito! | 5,52 MB | Adobe PDF | Ver/Abrir |