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https://hdl.handle.net/1822/74500
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Campo DC | Valor | Idioma |
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dc.contributor.author | Vilas Boas, Diana | por |
dc.contributor.author | Melo, Luís Daniel Rodrigues | por |
dc.date.accessioned | 2021-10-25T10:19:15Z | - |
dc.date.issued | 2021-02-13 | - |
dc.identifier.citation | Vilas Boas, Diana; Melo, Luís Daniel Rodrigues, Monitoring bacteriophage infection on bacterial cells using FISH. In Nuno F. Azevedo, Carina Almeida, Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts, Vol. Methods in Molecular Biology 2246, Springer US, 2021. ISBN: 978-1-0716-1115-9, 157-168 | por |
dc.identifier.isbn | 978-1-0716-1115-9 | por |
dc.identifier.issn | 1064-3745 | por |
dc.identifier.uri | https://hdl.handle.net/1822/74500 | - |
dc.description.abstract | The possibility of visualizing bacteriophage-host interactions through fluorescence in situ hybridization (FISH)-derived methods is gaining relevance in the last few years. These methods allow the possibility of discriminating between phage-infected and noninfected cells and the assessment of the different infection stages at the single cell level. In opposition to bacterial cells, the detection of phages is more challenging due to the low number of nucleic acid copies. However, by using a conserved region of the phage genome that is highly expressed during transcription, a FISH signal targeting phage DNA copies and mRNA transcripts can be easily visible inside the bacterial host cells. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Sci ence and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI 01-0145-FEDER-006684) and Project PTDC/SAU-PUB/29182/2017 (POCI-01-0145-FEDER-029182). This work was also supported by BioTecNorte operation (NORTE-01-0145- FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte. | por |
dc.language.iso | eng | por |
dc.publisher | Springer | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.relation | PTDC/SAU-PUB/29182/201 | por |
dc.rights | restrictedAccess | por |
dc.subject | Bacteriophage | por |
dc.subject | Detection | por |
dc.subject | Microscopy | por |
dc.subject | Phage-host interactions | por |
dc.title | Monitoring bacteriophage infection on bacterial cells using FISH | por |
dc.type | bookPart | por |
dc.peerreviewed | yes | - |
dc.relation.publisherversion | https://link.springer.com/book/10.1007/978-1-0716-1115-9 | por |
dc.comments | CEB54197 | por |
oaire.citationStartPage | 157 | por |
oaire.citationEndPage | 168 | por |
oaire.citationConferencePlace | New York, NY | - |
oaire.citationVolume | Methods in Molecular Biology 2246 | - |
dc.date.updated | 2021-10-25T08:44:47Z | - |
dc.identifier.doi | 10.1007/978-1-0716-1115-9_11 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 33576989 | por |
dc.subject.fos | Ciências Naturais::Ciências Biológicas | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Methods in Molecular Biology | por |
sdum.bookTitle | Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts | por |
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