Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75131
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Abreu, C. M. | por |
dc.contributor.author | Gasperini, Luca | por |
dc.contributor.author | Lago, Manuela Ermelinda Lopes | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Marques, A. P. | por |
dc.date.accessioned | 2022-01-03T17:00:12Z | - |
dc.date.available | 2022-01-03T17:00:12Z | - |
dc.date.issued | 2021-10 | - |
dc.date.submitted | 2021-12 | - |
dc.identifier.citation | Abreu C. M., Gasperini L., Lago M. E. L., Reis R. L., Marques A. P. Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages, Bioengineering and Translational Medicine, pp. e10195, doi:10.1002/btm2.10195, 2021 | por |
dc.identifier.issn | 2380-6761 | por |
dc.identifier.uri | https://hdl.handle.net/1822/75131 | - |
dc.description.abstract | Engineering complex tissues requires the use of advanced biofabrication techniques that allow the replication of the tissue's 3D microenvironment, architecture and cellular interactions. In the case of skin, the most successful strategies to introduce the complexity of hair follicle (HF) appendages have highlighted the importance of facilitating direct interaction between dermal papilla (DP) cells and keratinocytes (KCs) in organotypic skin models. In this work, we took advantage of microscopy-guided laser ablation (MGLA) to microfabricate a fibroblast-populated collagen hydrogel and create a subcompartment that guides the migration of KCs and lead their interaction with DP cells to recreate follicular structures. Upon definition of the processing parameters (laser incidence area and power), MGLA was used to create 3D microchannels from the surface of a standard organotypic human skin model up to the aggregates containing DP cells and KCs, previously incorporated into the dermal-like fibroblast-collagen layer. Analysis of the constructs showed that the fabricated microfeatures successfully guided the fusion between epidermal and aggregates keratinocytes, which differentiated into follicular-like structures within the organotypic human skin model, increasing its functionality. In summary, we demonstrate the fabrication of a highly structured 3D hydrogel-based construct using MGLA to attain a complex skin model bearing folliculoid structures, highlighting its potential use as an in vitro platform to study the mechanisms controlling HF development or for the screening of bioactive substances. | por |
dc.description.sponsorship | The authors would like to acknowledge the financial support from the Consolidator Grant “ECM_INK” (ERC-2016-COG-726061) and from FCT/MCTES (Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia, e Ensino Superior) grants PD/59/2013, PD/BD/113800/2015 (Carla Abreu), PD/169/2013 (Manuela E. L. Lago) and IF/00945/2014 (Alexandra P. Marques). The authors also acknowledge the financial support from the FSE/POCH (Fundo Social Europeu através do Programa Operacional do Capital Humano) under the scope of the NORTE-08-5369-FSE-000037 (Manuela E. L. Lago) grant. | por |
dc.language.iso | eng | por |
dc.publisher | John Wiley & Sons Ltd | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/726061/EU | por |
dc.relation | PD/169/2013 | por |
dc.relation | PD/BD/113800/2015 | por |
dc.relation | PD/59/ 2013 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | hair follicle | por |
dc.subject | In Vitro Model | por |
dc.subject | Laser ablation | por |
dc.subject | 3D tissue model | por |
dc.subject | biofabrication | por |
dc.subject | dermal papilla | por |
dc.subject | skin model | por |
dc.title | Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://aiche.onlinelibrary.wiley.com/doi/full/10.1002/btm2.10195 | por |
dc.comments | http://3bs.uminho.pt/node/20673 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 6 | por |
dc.date.updated | 2021-12-23T17:20:45Z | - |
dc.identifier.doi | 10.1002/btm2.10195 | por |
dc.subject.fos | Engenharia e Tecnologia::Outras Engenharias e Tecnologias | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Bioengineering & Translational Medicine | por |
oaire.version | VoR | por |
dc.identifier.articlenumber | e10195 | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20673-bioengineering-transla-med-2020-abreu-microscopy‐guided-laser-ablation-creation-complex-skin-models-.pdf | 3,07 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons