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https://hdl.handle.net/1822/7759
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Campo DC | Valor | Idioma |
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dc.contributor.author | Madeira, Pedro | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Macedo, Eugénia | - |
dc.contributor.author | Mikheeva, Larissa M. | - |
dc.contributor.author | Zaslavsky, Boris Y. | - |
dc.date.accessioned | 2008-03-31T21:20:48Z | - |
dc.date.available | 2008-03-31T21:20:48Z | - |
dc.date.issued | 2008-03 | - |
dc.identifier.citation | "Journal of Chromatography A". ISSN 0021-9673. 1185:1 (21 Mar. 2008) 85-92. | eng |
dc.identifier.issn | 0021-9673 | eng |
dc.identifier.uri | https://hdl.handle.net/1822/7759 | - |
dc.description.abstract | Phase diagrams were determined for aqueous two-phase systems (ATPSs) formed by different paired combinations of Dextran (Dex-75), Ficoll-70, polyethylene glycol (PEG-8000), hydroxypropyl starch (PES-100), and Ucon50HB5100 (a random copolymer of ethylene glycol and propylene glycol) all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, at 23 °C. Partition coefficients of a series of dinitrophenylated (DNP) amino acids with aliphatic side-chains were studied in all the ATPSs at particular polymer concentrations. Free energies of transfer of a methylene group between the coexisting phases, ΔG(CH2), were determined as measures of the difference between the hydrophobic character of the phases. Furthermore, partition coefficients of tryptophan (Trp) and its di- and tri-peptides and a set of p-nitrophenyl (NP)-monosaccharides were measured in all the two-phase systems, and the data obtained compared with the ΔG(CH2) values obtained in the systems. It was established that for eight out of 10 of two-phase systems of different polymer compositions the partition coefficients for Trp peptides correlate well with the ΔG(CH2) values. Similar correlations for NP-monosaccharides were valid for seven out of 10 two-phase systems. These observations indicate that the difference between the hydrophobic characters of the coexisting phases represented by the ΔG(CH2) value cannot be used as a single universal measure for comparison of the ATPSs of different polymer compositions. | eng |
dc.description.sponsorship | LSRE | eng |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) - SFRH/BD/18397/2004, FEDER/POCI/2010 | eng |
dc.language.iso | eng | eng |
dc.publisher | Elsevier B.V. | eng |
dc.relation | info:eu-repo/grantAgreement/FCT/POCI/60720/PT | - |
dc.rights | openAccess | eng |
dc.subject | Aqueous two-phase partitioning | eng |
dc.subject | Hydrophobicity | eng |
dc.subject | Amino acids | eng |
dc.subject | Tryptophan | eng |
dc.subject | Peptides | eng |
dc.subject | Monosaccharides | eng |
dc.title | ΔG(CH2) as solvent descriptor in polymer/polymer aqueous two-phase systems | eng |
dc.type | article | por |
dc.peerreviewed | yes | eng |
dc.relation.publisherversion | http://www.sciencedirect.com/science/journal/00219673 | eng |
sdum.number | 1 | eng |
sdum.pagination | 85-92 | eng |
sdum.publicationstatus | published | eng |
sdum.volume | 1185 | eng |
oaire.citationStartPage | 85 | por |
oaire.citationEndPage | 92 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 1185 | por |
dc.identifier.doi | 10.1016/j.chroma.2008.01.035 | por |
dc.identifier.pmid | 18258243 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Chromatography A | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Madeira_J_ChromA[2].pdf | 238,64 kB | Adobe PDF | Ver/Abrir |