Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78448
Título: | Silk fibroin/cholinium gallate-based architectures as therapeutic tools |
Autor(es): | Gomes, Joana M. Silva, Simone S. Fernandes, Emanuel Mouta Lobo, Flávia Cristina Marques Martín-Pastor, Manuel Taboada, Pablo Reis, R. L. |
Palavras-chave: | Anti-inflammatory properties Antioxidant properties Biocompatible ionic liquid Silk fibroin Cholinium gallate |
Data: | Mai-2022 |
Editora: | Elsevier 1 |
Revista: | Acta Biomaterialia |
Citação: | Gomes J. M., Silva S. S., Fernandes E. M., Lobo F. C. M., Martín-Pastor M., Taboada P., Reis R. L. Silk fibroin/cholinium gallate-based architectures as therapeutic tools, Acta Biomaterialia, pp. 1742-7061, doi:10.1016/j.actbio.2022.05.020, 2022 |
Resumo(s): | The combination of natural resources with biologically active biocompatible ionic liquids (Bio-IL) is presented as a combinatorial approach for developing tools to manage inflammatory diseases. Innovative biomedical solutions were constructed combining silk fibroin (SF) and Ch[Gallate], a Bio-IL with antioxidant and anti-inflammatory features, as freeze-dried 3D-based sponges. An evaluation of the effect of the Ch[Gallate] concentration (⠤3% w/v) on the SF/Ch[Gallate] sponges was studied. Structural changes observed on the sponges revealed that the Ch[Gallate] presence positively affected the β-sheet formation while not influencing the silk native structure, which was suggested by the FTIR and solid-state NMR results, respectively. Also, it was possible to modulate their mechanical properties, antioxidant activity and stability/degradation in an aqueous environment, by changing the Ch[Gallate] concentration. The architectures showed high water uptake ability and a weight loss that follows the controlled Ch[Gallate] release rate studied for 7 days. Furthermore, the sponges supported human adipose stem cells growth and proliferation, up to 7 days. TNF-α, IL-6 (pro-inflammatory) and IL-10 (anti-inflammatory) release quantification from a human monocyte cell line revealed a decrease in the pro-inflammatory cytokines concentrations in samples containing Ch[Gallate]. These outcomes encourage the use of the developed architectures as tissue engineering solutions, potentially targeting inflammation processes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/78448 |
DOI: | 10.1016/j.actbio.2022.05.020 |
ISSN: | 1742-7061 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S1742706122002884 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20781-1-s20-s1742706122002884-main.pdf Acesso restrito! | 3,57 MB | Adobe PDF | Ver/Abrir |