Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/80254
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Martins, Cátia D. F. | por |
dc.contributor.author | Raposo, M. Manuela M. | por |
dc.contributor.author | Costa, Susana P. G. | por |
dc.date.accessioned | 2022-10-19T20:08:59Z | - |
dc.date.available | 2022-10-19T20:08:59Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Martins, C.D.F.; Raposo, M.M.M.; Costa, S.P.G. A New Fluorogenic Substrate for Granzyme B Based on Fluorescence Resonance Energy Transfer. Chem. Proc. 2021, 3, 6. https://doi.org/10.3390/ecsoc-24-08311 | por |
dc.identifier.uri | https://hdl.handle.net/1822/80254 | - |
dc.description.abstract | The synthesis and characterization of a new fluorogenic substrate for granzyme B (GzmB) is reported. The substrate design was based on the fluorescence resonance energy transfer (FRET) principle using 5-(2′-aminoethyl)aminonaphthalene sulfonic acid (Edans) and 4-[[4′-(N,N-dimethylamino)phenyl]diazenyl]benzoic acid (Dabcyl) as a donor–acceptor pair, linked to a specific sequence for GzmB (AAD), with an additional amino acid as the anchoring point (K). The tetrapeptide was synthesized by microwave-assisted solid-phase peptide synthesis (MW-SPPS) and coupled to Dabcyl and Edans at its N- and C-termini, respectively. The obtained probe was purified by semi-preparative HPLC and characterized by NMR, UV/Vis absorption and fluorescence spectroscopy and mass spectrometry. | por |
dc.description.sponsorship | The authors acknowledge Fundação para a Ciência e Tecnologia—FCT (Portugal), for funding through CQUM (UIDB/00686/2020) and project PTDC/QUI-COL/28052/2017. The NMR spectrometer Bruker Avance III 400 is part of the National NMR Network and was purchased within the framework of the National Program for Scientific Re-equipment, contract REDE/1517/RMN/2005 with funds from POCI 2010 (FEDER) and FCT. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00686%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FQUI-COL%2F28052%2F2017/PT | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | donor–acceptor pair | por |
dc.subject | FRET | por |
dc.subject | granzyme B | por |
dc.subject | fluorescent probes | por |
dc.subject | peptides | por |
dc.subject | synthesis | por |
dc.title | A new fluorogenic substrate for granzyme B based on fluorescence resonance energy transfer | por |
dc.type | conferencePaper | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/2673-4583/3/1/6 | por |
oaire.citationConferenceDate | 15 nov.- 15 Dez. 2020 | por |
sdum.event.title | 24th International Electronic Conference on Synthetic Organic Chemistry | por |
sdum.event.type | conference | por |
dc.identifier.eissn | 2673-4583 | - |
dc.identifier.doi | 10.3390/ecsoc-24-08311 | por |
dc.subject.fos | Ciências Naturais::Ciências Químicas | por |
sdum.journal | Chemistry Proceedings | - |
oaire.version | VoR | por |
dc.subject.ods | Saúde de qualidade | por |
Aparece nas coleções: | CDQuim - Comunicações e Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Chem. Proc. 2021, 3, 6.pdf | artigo em livro de atas | 411,15 kB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons