Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/81006
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Campo DC | Valor | Idioma |
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dc.contributor.author | Gimondi, Sara | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Ferreira, Helena | por |
dc.contributor.author | Neves, N. M. | por |
dc.date.accessioned | 2022-12-07T10:42:16Z | - |
dc.date.issued | 2022-04 | - |
dc.date.submitted | 2022-12 | - |
dc.identifier.citation | Gimondi S., Reis R. L., Ferreira H., Neves N. M. Microfluidic-driven mixing of high molecular weight polymeric complexes for precise nanoparticle downsizing, Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 43, doi:10.1016/j.nano.2022.102560, 2022 | por |
dc.identifier.issn | 1549-9634 | por |
dc.identifier.uri | https://hdl.handle.net/1822/81006 | - |
dc.description.abstract | Chitosan (CHIT) and hyaluronic acid (HA) are two polysaccharides (PSs) with high value in several biomedical applications. In this study, we present a microfluidic method to synthetize CHIT-HA NPs to overcome the disadvantages of the dropwise approach generally used for nanoprecipitation of polyelectrolyte complexes. The proposed microfluidic approach enables to generate monodisperse suspensions of NPs with ≈100 nm of size compared to the dropwise method that generated ≈2 times bigger NPs. Finally, we evaluated the potential of obtained NPs in an inflammatory scenario. The treatment with NPs led to the reduction of the main inflammatory molecules produced by macrophages (PGE2, IL-6, IL-8, MCAF and TNF-α) and fibroblasts (IL-1 α, PGE2, TNF-α) stimulated with lipopolysaccharide or conditioned medium, respectively. This study demonstrates that our approach can be used to enhance the synthesis of nanocarriers based on bioactive macromolecules | por |
dc.description.sponsorship | The authors would like to thank funding that allowed to carry out this work, namely the Fundação para a Ciência e a Tecnologia (FCT) for the S. Gimondi fellowship (PD/BD/143140/2019) and PATH program (PD/00169/2013). This work was also supported by Cells4_IDs (PTDC/BTM-SAL/28882/2017) and the NORTE 2020 Structured Project, co-funded by Norte2020 (NORTE-01-0145-FEDER-000021). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F143140%2F2019/PT | por |
dc.relation | PD/00169/2013 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBTM-SAL%2F28882%2F2017/PT | por |
dc.rights | closedAccess | por |
dc.subject | Chitosan | por |
dc.subject | Hyaluronic acid | por |
dc.subject | Inflammation | por |
dc.subject | Microfluidics | por |
dc.subject | Nanoparticle size | por |
dc.title | Microfluidic-driven mixing of high molecular weight polymeric complexes for precise nanoparticle downsizing | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://doi.org/10.1016/j.nano.2022.102560 | por |
dc.comments | http://3bs.uminho.pt/node/20867 | por |
oaire.citationVolume | 43 | por |
dc.date.updated | 2022-12-07T09:07:39Z | - |
dc.identifier.doi | 10.1016/j.nano.2022.102560 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 35417772 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Nanomedicine: Nanotechnology, Biology, and Medicine | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20867-05-microfluidic-high-molecular-weight-nps-downsizing.pdf Acesso restrito! | 1,7 MB | Adobe PDF | Ver/Abrir |