Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/83602
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Pedrosa, Ana Rita Silva | por |
dc.contributor.author | Campos, Jonas Oliveira | por |
dc.contributor.author | Fernandes, Aline Marie | por |
dc.contributor.author | Silva, Miguel | por |
dc.contributor.author | Calçada, Carla Sofia Martins | por |
dc.contributor.author | Marote, Ana Maria Franco Aveiro | por |
dc.contributor.author | Martinho, Olga | por |
dc.contributor.author | Veiga, Maria Isabel | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.contributor.author | Salgado, A. J. | por |
dc.contributor.author | Ferreira, Pedro Eduardo Mendes | por |
dc.date.accessioned | 2023-03-28T15:13:18Z | - |
dc.date.available | 2023-03-28T15:13:18Z | - |
dc.date.issued | 2023-03-16 | - |
dc.identifier.citation | Pedrosa, Ana; Campos, Jonas; Fernandes, Aline Marie; Silva, Miguel; Calçada, Carla; Marote, Ana; Martinho, Olga; Veiga, Maria Isabel; Rodrigues, Lígia R.; Salgado, António José; Ferreira, Pedro Eduardo, Cerebral malaria model applying human brain organoids. Cells, 12(7), 984, 2023 | por |
dc.identifier.uri | https://hdl.handle.net/1822/83602 | - |
dc.description.abstract | Neural injuries in cerebral malaria patients are a significant cause of morbidity and mortality. Nevertheless, a comprehensive research approach to study this issue is lacking, so herein we propose an in vitro system to study human cerebral malaria using cellular approaches. Our first goal was to establish a cellular system to identify the molecular alterations in human brain vasculature cells that resemble the blood–brain barrier (BBB) in cerebral malaria (CM). Through transcriptomic analysis, we characterized specific gene expression profiles in human brain microvascular endothelial cells (HBMEC) activated by the Plasmodium falciparum parasites. We also suggest potential new genes related to parasitic activation. Then, we studied its impact at brain level after Plasmodium falciparum endothelial activation to gain a deeper understanding of the physiological mechanisms underlying CM. For that, the impact of HBMEC-P. falciparum-activated secretomes was evaluated in human brain organoids. Our results support the reliability of in vitro cellular models developed to mimic CM in several aspects. These systems can be of extreme importance to investigate the factors (parasitological and host) influencing CM, contributing to a molecular understanding of pathogenesis, brain injury, and dysfunction. | por |
dc.description.sponsorship | This research was funded by National funds through the Foundation for Science and Technology (FCT) SFRH/BD/131540/2017, SFRH/BD/5813/2020, COVID/BD/152416/2022 and UMINHO/BIM-CNCG/2022/143. This work has been funded by ICVS Scientific Microscopy Platform, member of the national infrastructure PPBI - Portuguese Platform of Bioimaging (PPBIPOCI-01-0145-FEDER-022122; by National funds, through the FCT—project UIDB/50026/2020 and UIDP/50026/2020. Moreover, this work was funded by IF/00143/2015/CP1294/CT0001, PTDC/SAU-PAR/2766/2021 and UIDB/04469/2020. O.M. is funded by the project NORTE-01- 0247-FEDER-045914, supported by POFC–COMPETE and FCT, under the programs PT2020 and NORTE2020. M.I.V. thanks FCT for her contract funding provided through 2020.03113.CEECIND. | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F131540%2F2017/PT | por |
dc.relation | SFRH/BD/5813/2020 | por |
dc.relation | COVID/BD/152416/2022 | por |
dc.relation | UMINHO/BIM-CNCG/2022/143 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50026%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00143%2F2015%2FCP1294%2FCT0001/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PAR%2F2766%2F2021/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Cerebral malaria | por |
dc.subject | Brain organoids | por |
dc.subject | Transcriptome | por |
dc.subject | Human iPSCs | por |
dc.subject | Secretome | por |
dc.subject | HBMEC activation | por |
dc.title | Cerebral malaria model applying human brain organoids | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/journal/cells | por |
dc.comments | CEB56129 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 31 | por |
oaire.citationIssue | 7 | por |
oaire.citationConferencePlace | Switzerland | - |
oaire.citationVolume | 12 | por |
dc.date.updated | 2023-03-25T11:14:29Z | - |
dc.identifier.eissn | 2073-4409 | por |
dc.identifier.doi | 10.3390/cells12070984 | por |
dc.identifier.pmid | 37048057 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Cells | por |
oaire.version | VoR | por |
dc.identifier.articlenumber | 984 | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56129_1.pdf | 5,76 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons