Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/86538
Título: | Functional and sequence-based metagenomics to uncover carbohydrate-degrading enzymes from composting samples |
Autor(es): | Pereira, Cátia Sofia Santos Sousa, Joana Costa, Ângela Maria Araújo Santos, A. O. Rito, Teresa Soares, Pedro Franco-Duarte, Ricardo Silvério, Sara Isabel Cruz Rodrigues, L. R. |
Palavras-chave: | CAZymes Composting Glycoside hydrolases Lignocellulose Metagenomics |
Data: | 7-Jul-2023 |
Editora: | Springer Nature |
Revista: | Applied Microbiology and Biotechnology |
Citação: | Cátia S. Pereira; Sousa, Joana; Costa, Ângela; Santos, A. O.; Rito, Teresa; Soares, Pedro; Franco-Duarte, Ricardo; Silvério, Sara C.; Rodrigues, Lígia R., Functional and sequence-based metagenomics to uncover carbohydrate-degrading enzymes from composting samples. Applied Microbiology and Biotechnology, 107, 5379-5401, 2023 |
Resumo(s): | The renewable, abundant, and low-cost nature of lignocellulosic biomass can play an important role in the sustainable production of bioenergy and several added-value bioproducts, thus providing alternative solutions to counteract the global energetic and industrial demands. The efficient conversion of lignocellulosic biomass greatly relies on the catalytic activity of carbohydrate-active enzymes (CAZymes). Finding novel and robust biocatalysts, capable of being active under harsh industrial conditions, is thus imperative to achieve an economically feasible process. In this study, thermophilic compost samples from three Portuguese companies were collected, and their metagenomic DNA was extracted and sequenced through shotgun sequencing. A novel multi-step bioinformatic pipeline was developed to find CAZymes and characterize the taxonomic and functional profiles of the microbial communities, using both reads and metagenome-assembled genomes (MAGs) as input. The samples' microbiome was dominated by bacteria, where the classes Gammaproteobacteria, Alphaproteobacteria, and Balneolia stood out for their higher abundance, indicating that the degradation of compost biomass is mainly driven by bacterial enzymatic activity. Furthermore, the functional studies revealed that our samples are a rich reservoir of glycoside hydrolases (GH), particularly of GH5 and GH9 cellulases, and GH3 oligosaccharide-degrading enzymes. We further constructed metagenomic fosmid libraries with the compost DNA and demonstrated that a great number of clones exhibited $\beta$-glucosidase activity. The comparison of our samples with others from the literature showed that, independently of the composition and process conditions, composting is an excellent source of lignocellulose-degrading enzymes. To the best of our knowledge, this is the first comparative study on the CAZyme abundance and taxonomic/functional profiles of Portuguese compost samples. |
Tipo: | Artigo |
Descrição: | The online version contains supplementary material available at: https://doi.org/10.1007/s00253-023-12627-9. |
URI: | https://hdl.handle.net/1822/86538 |
DOI: | 10.1007/s00253-023-12627-9 |
ISSN: | 01757598 |
Versão da editora: | http://www.springer.com/chemistry/biotechnology/journal/253 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series CBMA - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_56344_1.pdf | 6,38 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons