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https://hdl.handle.net/1822/86885
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Campo DC | Valor | Idioma |
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dc.contributor.author | França, Ângela Maria Oliveira Sousa | por |
dc.contributor.author | Cerca, Nuno | por |
dc.date.accessioned | 2023-10-16T12:29:36Z | - |
dc.date.issued | 2023-08-23 | - |
dc.identifier.citation | França, A., Cerca, N. (2023). Gene Expression Quantification from Pathogenic Bacterial Biofilms by Quantitative PCR. In: Domingues, L. (eds) PCR. Methods in Molecular Biology, vol 2967. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3358-8_11 | por |
dc.identifier.isbn | 978-1-0716-3357-1 | por |
dc.identifier.issn | 1064-3745 | por |
dc.identifier.uri | https://hdl.handle.net/1822/86885 | - |
dc.description.abstract | Quantitative PCR (qPCR) is one of the most used techniques to quantify gene expression in bacterial biofilms due to its easiness, sensitivity, and robustness. However, several practical aspects need to be considered to obtain accurate and reliable results. Here, we describe a detailed and optimized protocol to quantify mRNA transcripts from bacterial biofilms using qPCR, including pieces of advice to improve RNA quality, which ultimately increases the accuracy, consistency, and relevance of gene expression data. | por |
dc.description.sponsorship | This work was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the unit (UIDB/04469/2020), EXPL/BIA-MIC/0032/2021 and EXPL/SAU-INF/1000/2021. | por |
dc.language.iso | eng | por |
dc.publisher | Springer | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIA-MIC%2F0032%2F2021/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FSAU-INF%2F1000%2F2021/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Bacteria | por |
dc.subject | Biofilms | por |
dc.subject | Sample collection | por |
dc.subject | RNA isolation | por |
dc.subject | DNase treatment | por |
dc.subject | RNA quality | por |
dc.subject | Complementary DNA synthesis | por |
dc.subject | qPCR | por |
dc.title | Gene expression quantification from pathogenic bacterial biofilms by quantitative PCR | por |
dc.type | bookPart | por |
dc.peerreviewed | yes | - |
dc.relation.publisherversion | https://link.springer.com/protocol/10.1007/978-1-0716-3358-8_11 | por |
dc.comments | CEB56463 | por |
oaire.citationStartPage | 133 | por |
oaire.citationEndPage | 149 | por |
oaire.citationConferencePlace | New York, New York, NY | - |
oaire.citationVolume | MIMB 2967 | - |
dc.date.updated | 2023-10-16T11:51:26Z | - |
dc.identifier.doi | 10.1007/978-1-0716-3358-8_11 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.eisbn | 978-1-0716-3358-8 | por |
dc.identifier.pmid | 37608108 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.journal | Methods in Molecular Biology | por |
sdum.bookTitle | PCR: Methods and Protocols, Second Edition | por |
oaire.version | VoR | por |
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