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https://hdl.handle.net/1822/91506
Título: | Using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) to detect Campylobacter spp. in food samples |
Autor(es): | Oliveira, Ricardo Bruno Costa Barbosa, Ana Sousa, Mário Azevedo, Nuno Filipe Cerqueira, Laura Almeida, Carina Manuela Fernandes |
Palavras-chave: | Foodborne illnesses PNA-FISH Pre-enrichment Food samples |
Data: | 15-Abr-2024 |
Editora: | Elsevier 1 |
Revista: | LWT-Food Science and Technology |
Citação: | Oliveira, R.; Barbosa, Ana; Sousa, Mário; Azevedo, Nuno Filipe; Cerqueira, Laura; Almeida, Carina, Using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) to detect Campylobacter spp. in food samples. LWT-Food Science and Technology, 198(115922), 2024 |
Resumo(s): | Foodborne diseases have a considerable negative impact on socioeconomic development globally and are an important cause of morbidity and mortality. Among the foodborne bacterial pathogens, Campylobacter spp. is recognized as the leading cause of foodborne illness. Fluorescent in situ hybridization using nucleic acid mimics (NAM-FISH) as probes, in particular peptide nucleic acid (PNA) probes, is a molecular technique that has emerged as an essential and resourceful tool for bacterial detection. Here, we applied a PNA-FISH methodology, including pre-enrichment culture, for the specific detection of Campylobacter spp. in food matrices, more specifically fresh raw broiler meat and fresh raw pork. New PNA probes, including a blocker probe, have been designed for a 23S rRNA sequence. The PNA-FISH technique presented sensitivity and specificity values of 92.0% and 96.9%, respectively. In food matrcies, the best detection condition was achieved with a pre-enrichment of 48h in Bolton broth, allowing a detection limit of 1CFU/25g. Compared to the ISO 10272-1:2017 reference method, this methodology showed similar performance in food matrices. The present study revealed that the developed PNA-FISH method is a promising alternative for detecting Campylobacter spp. in food samples. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/91506 |
DOI: | 10.1016/j.lwt.2024.115922 |
ISSN: | 0023-6438 |
Versão da editora: | https://www.journals.elsevier.com/lwt |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57764_1.pdf | 1,26 MB | Adobe PDF | Ver/Abrir |