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https://hdl.handle.net/1822/44457
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Campo DC | Valor | Idioma |
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dc.contributor.author | Monteiro, I. P. | por |
dc.contributor.author | Gabriel, D. | por |
dc.contributor.author | Timko, B. P. | por |
dc.contributor.author | Hashimoto, M. | por |
dc.contributor.author | Karajanagi, S. | por |
dc.contributor.author | Tong, R. | por |
dc.contributor.author | Marques, A. P. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Kohane, D. S. | por |
dc.date.accessioned | 2017-01-25T10:47:41Z | - |
dc.date.available | 2017-01-25T10:47:41Z | - |
dc.date.issued | 2014-09 | - |
dc.date.submitted | 2016-04 | - |
dc.identifier.citation | Monteiro I. P., Gabriel D., Timko B. P., Hashimoto M., Karajanagi S., Tong R., Marques A. P., Reis R. L., Kohane D. S. A two-component pre-seeded dermal-epidermal scaffold, Acta Biomaterialia, Vol. 10, Issue 12, pp. 4928-4938, doi:10.1016/j.actbio.2014.08.029, 2014 | por |
dc.identifier.issn | 1742-7061 | por |
dc.identifier.uri | https://hdl.handle.net/1822/44457 | - |
dc.description.abstract | We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment. | por |
dc.description.sponsorship | D.S.K. acknowledges funding from the Biotechnology Research Endowment from the Department of Anesthesiology at Boston Children's Hospital. I.P.M. acknowledges the Portuguese Foundation for Science and Technology for the grant BD/39396/2007 and the MIT-Portugal Program. D.G. acknowledges the Swiss National Science Foundation for a post-doctoral fellowship (PBGEP3-129111). B.P.T. acknowledges an NIR Ruth L. Kirschstein National Research Service Award (F32GM096546). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Skin defects | por |
dc.subject | Bilayered | por |
dc.subject | Hyaluronic acid | por |
dc.subject | Amine–aldehyde bonding | por |
dc.title | A two-component pre-seeded dermal-epidermal scaffold | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S1742706114003742 | por |
dc.comments | http://3bs.uminho.pt/node/18709 | por |
oaire.citationStartPage | 4928 | por |
oaire.citationEndPage | 4938 | por |
oaire.citationIssue | 4937 | por |
oaire.citationTitle | Acta Biomaterialia | por |
oaire.citationVolume | 4929 | por |
dc.date.updated | 2017-01-11T15:13:46Z | - |
dc.identifier.doi | 10.1016/j.actbio.2014.08.029 | por |
dc.identifier.pmid | 25192821 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Acta Biomaterialia | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18709-Monteiro IP.pdf | 2,35 MB | Adobe PDF | Ver/Abrir |