Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/44457
Título: | A two-component pre-seeded dermal-epidermal scaffold |
Autor(es): | Monteiro, I. P. Gabriel, D. Timko, B. P. Hashimoto, M. Karajanagi, S. Tong, R. Marques, A. P. Reis, R. L. Kohane, D. S. |
Palavras-chave: | Skin defects Bilayered Hyaluronic acid Amine–aldehyde bonding |
Data: | Set-2014 |
Editora: | Elsevier 1 |
Revista: | Acta Biomaterialia |
Citação: | Monteiro I. P., Gabriel D., Timko B. P., Hashimoto M., Karajanagi S., Tong R., Marques A. P., Reis R. L., Kohane D. S. A two-component pre-seeded dermal-epidermal scaffold, Acta Biomaterialia, Vol. 10, Issue 12, pp. 4928-4938, doi:10.1016/j.actbio.2014.08.029, 2014 |
Resumo(s): | We have developed a bilayered dermal-epidermal scaffold for application in the treatment of full-thickness skin defects. The dermal component gels in situ and adapts to the lesion shape, delivering human dermal fibroblasts in a matrix of fibrin and cross-linked hyaluronic acid modified with a cell adhesion-promoting peptide. Fibroblasts were able to form a tridimensional matrix due to material features such as tailored mechanical properties, presence of protease-degradable elements and cell-binding ligands. The epidermal component is a robust membrane containing cross-linked hyaluronic acid and poly-l-lysine, on which keratinocytes were able to attach and to form a monolayer. Amine-aldehyde bonding at the interface between the two components allows the formation of a tightly bound composite scaffold. Both parts of the scaffold were designed to provide cell-type-specific cues to allow for cell proliferation and form a construct that mimics the skin environment. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/44457 |
DOI: | 10.1016/j.actbio.2014.08.029 |
ISSN: | 1742-7061 |
Versão da editora: | http://www.sciencedirect.com/science/article/pii/S1742706114003742 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18709-Monteiro IP.pdf | 2,35 MB | Adobe PDF | Ver/Abrir |