Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/50943
Título: | A new method for yeast phagocytosis analysis by flow cytometry |
Autor(es): | Carneiro, Maria Catarina Santos Vaz, Catarina Oliveira Pais, Célia Sampaio, Paula Pereira, Joana Isabel Carvalho |
Palavras-chave: | Candida spp Flow cytometry Sytox green Propidium iodide Phagocytosis PAMPs |
Data: | 2014 |
Editora: | Elsevier |
Revista: | Journal of Microbiological Methods |
Resumo(s): | Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All rights reserved. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/50943 |
DOI: | 10.1016/j.mimet.2014.03.013 |
ISSN: | 0167-7012 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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(2014) J Microbiol Methods .pdf | 1,48 MB | Adobe PDF | Ver/Abrir |