Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75262
Título: | A new PNA-FISH probe targeting Fannyhessea vaginae |
Autor(es): | Sousa, Lúcia Filipa Guimarães Vieira Castro, Joana Isabel Reis França, Ângela Maria Oliveira Sousa Almeida, Carina Muzny, C. A. Cerca, Nuno |
Palavras-chave: | Fannyhessea vaginae Gardnerella vaginalis bacterial vaginosis fluorescence in situ hybridization (FISH) peptide nucleic acid (PNA) |
Data: | 18-Nov-2021 |
Editora: | Frontiers Media S. A. |
Revista: | Frontiers in Cellular and Infection Microbiology |
Citação: | Sousa, Lúcia; Castro, Joana; França, Angela; Almeida, Carina; Muzny, C. A.; Cerca, Nuno, A new PNA-FISH probe targeting Fannyhessea vaginae. Frontiers in Cellular and Infection Microbiology, 11(779376), 2021 |
Resumo(s): | Bacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age and has been associated with serious health complications, mainly in pregnant women. It is characterized by a decrease in the number of Lactobacillus species in the healthy vaginal microbiota and an overgrowth of strict and facultative anaerobic bacteria that develop a polymicrobial biofilm. Despite over 60 years of research investigating BV, its etiology is not fully understood. Gardnerella spp. is a crucial microorganism that contributes to the formation of the biofilm and the development of BV, but the role of other BV-associated bacteria is not clear. Nevertheless, Fannyhessea vaginae (previously known as Atopobium vaginae) is a highly specific species for BV, and co-colonization with Gardnerella is thought to be a very specific diagnostic marker. The diagnosis of BV still presents some limitations, since currently used methods often fail to accurately detect BV. This work aims to develop a novel peptide nucleic acid (PNA) probe targeting F. vaginae. This probe was further validated in a multiplex assay, which included a Gardnerella-specific PNA probe, as a possible method for diagnosis of BV, and was compared with quantification by qPCR. The new PNA probe showed excellent sensitivity and specificity and could discriminate F. vaginae-Gardnerella biofilms, confirming the potential to be used for the detection of BV-associated pathogens. |
Tipo: | Artigo |
Descrição: | The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021.779376/full#supplementary-material |
URI: | https://hdl.handle.net/1822/75262 |
DOI: | 10.3389/fcimb.2021.779376 |
ISSN: | 2235-2988 |
Versão da editora: | https://www.frontiersin.org/journals/cellular-and-infection-microbiology |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55110_1.pdf | 3,14 MB | Adobe PDF | Ver/Abrir |