Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78411
Título: | Antimicrobial activity of a bacterial nanocellulose film functionalized with Nisin Z for prospective burn wounds treatment |
Autor(es): | Melro, Liliana Sofia Silva Ferreira Pinto Tavares, Tânia Daniela Eugénio Padrão, Jorge Dourado, Fernando Gama, F. M. Silva, Carla Manuela Costa Antunes, Joana Carolina Simões Felgueiras, Helena Prado Zille, Andrea |
Palavras-chave: | Textile Antimicrobial peptides Bacterial nanocellulose Wound dressing Nisin Z Burn wounds |
Data: | 2022 |
Resumo(s): | Burn wounds can lead to numerous severe complications including bacterial infections causing patient morbidity and mortality, mostly in low- and middle-income countries. The considerable increase of the microbial resistance against traditional antibiotics is leading towards alternative strategies to treat bacterial infections. Nisin Z is an antimicrobial peptide which exhibits a significant antibacterial activity against Gram-positive bacteria. Its efficacy against Gram-negative bacteria is limited, nonetheless it can be improved with the addition of surfactants, such as ethylenediaminetetraacetic acid (EDTA). The incorporation of peptide and other biomolecules within a biopolymer matrix provides protection maintaining their antimicrobial potential. Bacterial nanocellulose (BNC) has been widely used as wound dressings. Its impressive water retention capacity (> 99 %) and porosity are beneficial to manage wounds due to its potential to absorb exudates, providing a breathable and humid environment. In this work, the functionalization of BNC with Nisin Z (BNC-NZ) via vacuum filtration is reported. The entrapment of the peptide inside the BNC films was confirmed through morphological characterization using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectrometry. Typical absorbance peaks of Nisin Z are easily identifiable at 1647 cm-1 (amide group) and 1520 cm-1 (bending of primary amines). Thermal Gravimetric Analysis (TGA) suggested that Nisin Z did not interfere with the BNC matrix. The antimicrobial activity of Nisin Z against five of the most common bacteria found in burn wounds was verified by Minimum Bactericidal Concentration (MBC) ranging 8.0-256.0 µg/mL. Agar Diffusion and Shake Flask methods revealed the potential of BNC-NZ for prospective applications in burn wound dressings. |
Tipo: | Poster em conferência |
URI: | https://hdl.handle.net/1822/78411 |
Versão da editora: | https://sciforum.net/paper/view/12708 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Comunicações em congressos internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
slides.pdf | 1,62 MB | Adobe PDF | Ver/Abrir |