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TitleAntimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment
Author(s)Melro, Liliana
Tavares, Tânia D.
Padrão, Jorge
Dourado, Fernando
Gama, F. M.
Silva, C.
Antunes, Joana C.
Felgueiras, Helena Prado
Zille, Andrea
Keywordsantimicrobial peptides
Nisin Z
bacterial nanocellulose
wound dressing
Issue date15-Jun-2022
JournalMedical Sciences Forum
CitationMelro, Liliana; Tavares, Tânia D.; Padrão, Jorge; Dourado, Fernando; Gama, F. M.; Silva, Carla; Antunes, Joana C.; Felgueiras, Helena P.; Zille, Andrea, Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment. Medical Sciences Forum. Vol. 12(1), MDPI AG, 1, 2022.
Abstract(s)Burn wounds can lead to numerous severe complications including bacterial infections causing patient morbidity and mortality, mostly in low- and middle-income countries. The considerable increase in microbial resistance against traditional antibiotics is leading towards alternative strategies to treat bacterial infections. Nisin Z is an antimicrobial peptide which exhibits a significant antibacterial activity against Gram-positive bacteria. Its efficacy against Gram-negative bacteria is limited, nonetheless it can be improved with the addition of surfactants, such as ethylenediaminetetraacetic acid (EDTA). The incorporation of peptide and other biomolecules within a biopolymer matrix provides protection maintaining their antimicrobial potential. Bacterial nanocellulose (BNC) has been widely used as wound dressings. Its impressive water retention capacity (>99%) and porosity are beneficial to manage wounds due to its potential to absorb exudates, providing a breathable and humid environment. In this work, the functionalization of BNC with Nisin Z (BNC-NZ) via vacuum filtration is reported. The entrapment of the peptide inside the BNC films was confirmed through morphological characterization using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrometry. Typical absorbance peaks of Nisin Z are easily identifiable at 1647 cm−1 (amide group) and 1520 cm−1 (bending of primary amines). Thermal gravimetric analysis (TGA) suggested that Nisin Z did not interfere with the BNC matrix. The antimicrobial activity of Nisin Z against five of the most common bacteria found in burn wounds was verified by minimum bactericidal concentration (MBC) ranging 8.0–256.0 µg/mL. Agar diffusion and shake flask methods revealed the potential of BNC-NZ for prospective applications in burn wound dressings.
DescriptionSupplementary Materials: The following are available online at .3390/eca2022-12708/s1
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
DET/2C2T - Comunicações em congressos internacionais com arbitragem científica

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