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https://hdl.handle.net/1822/80976
Título: | Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment |
Autor(es): | Melro, Liliana Tavares, Tânia D. Padrão, Jorge Dourado, Fernando Gama, F. M. Silva, C. Antunes, Joana C. Felgueiras, Helena Prado Zille, Andrea |
Palavras-chave: | antimicrobial peptides Nisin Z bacterial nanocellulose wound dressing |
Data: | 15-Jun-2022 |
Editora: | MDPI |
Revista: | Medical Sciences Forum |
Citação: | Melro, Liliana; Tavares, Tânia D.; Padrão, Jorge; Dourado, Fernando; Gama, F. M.; Silva, Carla; Antunes, Joana C.; Felgueiras, Helena P.; Zille, Andrea, Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment. Medical Sciences Forum. Vol. 12(1), MDPI AG, 1, 2022. |
Resumo(s): | Burn wounds can lead to numerous severe complications including bacterial infections causing patient morbidity and mortality, mostly in low- and middle-income countries. The considerable increase in microbial resistance against traditional antibiotics is leading towards alternative strategies to treat bacterial infections. Nisin Z is an antimicrobial peptide which exhibits a significant antibacterial activity against Gram-positive bacteria. Its efficacy against Gram-negative bacteria is limited, nonetheless it can be improved with the addition of surfactants, such as ethylenediaminetetraacetic acid (EDTA). The incorporation of peptide and other biomolecules within a biopolymer matrix provides protection maintaining their antimicrobial potential. Bacterial nanocellulose (BNC) has been widely used as wound dressings. Its impressive water retention capacity (>99%) and porosity are beneficial to manage wounds due to its potential to absorb exudates, providing a breathable and humid environment. In this work, the functionalization of BNC with Nisin Z (BNC-NZ) via vacuum filtration is reported. The entrapment of the peptide inside the BNC films was confirmed through morphological characterization using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrometry. Typical absorbance peaks of Nisin Z are easily identifiable at 1647 cm−1 (amide group) and 1520 cm−1 (bending of primary amines). Thermal gravimetric analysis (TGA) suggested that Nisin Z did not interfere with the BNC matrix. The antimicrobial activity of Nisin Z against five of the most common bacteria found in burn wounds was verified by minimum bactericidal concentration (MBC) ranging 8.0–256.0 µg/mL. Agar diffusion and shake flask methods revealed the potential of BNC-NZ for prospective applications in burn wound dressings. |
Tipo: | Resumo em ata de conferência |
Descrição: | Supplementary Materials: The following are available online at https://www.mdpi.com/article/10 .3390/eca2022-12708/s1 |
URI: | https://hdl.handle.net/1822/80976 |
DOI: | 10.3390/eca2022-12708 |
ISSN: | 2673-9992 |
Versão da editora: | https://www.mdpi.com/2673-9992/12/1/1 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DET/2C2T - Comunicações em congressos internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55928_1.pdf | 216,23 kB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons