Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/85220
Título: | Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae |
Autor(es): | Pozdniakova, T. A. Cruz, João P. Silva, Paulo César Azevedo, Flávio Parpot, Pier Domingues, Maria Rosario Carlquist, Magnus Johansson, Björn |
Palavras-chave: | Saccharomyces cerevisiae E. coli Fatty acid synthase FASI FASII Metabolic engineering |
Data: | Dez-2023 |
Editora: | Elsevier B.V. |
Revista: | Metabolic Engineering Communications |
Citação: | Pozdniakova, T. A.; Cruz, J. P.; Silva, P. C.; Azevedo, F.; Parpot, Pier; Domingues, M. R.; Carlquist, M.; Johansson, B., Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae. Metabolic Engineering Communications, 17(e00224), 2023 |
Resumo(s): | Fatty acids are produced by eukaryotes like baker’s yeast Saccharomyces cerevisiae mainly using a large multifunctional type I fatty acid synthase (FASI) where seven catalytic steps and a carrier domain are shared between one or two protein subunits. While this system may offer efficiency in catalysis, only a narrow range of fatty acids are produced. Prokaryotes, chloroplasts and mitochondria rely instead on a FAS type II (FASII) where each catalytic step is carried out by a monofunctional enzyme encoded by a separate gene. FASII is more flexible and capable of producing a wider range of fatty acid structures, such as the direct production of unsaturated fatty acids. An efficient FASII in the preferred industrial organism S. cerevisiae could provide a platform for developing sustainable production of specialized fatty acids. We functionally replaced either yeast FASI genes (FAS1 or FAS2) with a FASII consisting of nine genes from Escherichia coli (acpP, acpS and fab -A, -B, -D, -F, -G, -H, -Z) as well as three from Arabidopsis thaliana (MOD1, FATA1 and FATB). The genes were expressed from an autonomously replicating multicopy vector assembled using the Yeast Pathway Kit for in-vivo assembly in yeast. Two rounds of adaptation led to a strain with a maximum growth rate (μmax) of 0.19 h− 1 without exogenous fatty acids, twice the growth rate previously reported for a comparable strain. Additional copies of the MOD1 or fabH genes resulted in cultures with higher final cell densities and three times higher lipid content compared to the control. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/85220 |
DOI: | 10.1016/j.mec.2023.e00224 |
ISSN: | 2214-0301 |
Versão da editora: | http://www.journals.elsevier.com/metabolic-engineering-communications |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_56290_1.pdf | 1,68 MB | Adobe PDF | Ver/Abrir |