Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/88381
Título: | A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks |
Autor(es): | Simões, Lúcia Chaves Oliveira, Isabel Borges, Anabela Gomes, Inês Bezerra Simões, Manuel |
Palavras-chave: | agitation hydrodynamic force microbial growth shake flasks standardization |
Data: | Dez-2023 |
Editora: | American Society for Microbiology |
Revista: | Journal of Microbiology & Biology Education |
Citação: | Simões, L. C., Oliveira, I., Borges, A., Gomes, I. B., & Simões, M. (2023, December 14). A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks. (L. J. MacDonald, Ed.), Journal of Microbiology & Biology Education. American Society for Microbiology. http://doi.org/10.1128/jmbe.00099-23 |
Resumo(s): | Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size. Liquid agitation is implemented inherently to increase aeration, substrate transfer to the cells, and prevent sedimentation, disregarding the role of hydrodynamics in microbial growth and metabolism. Here, we present a simple approach to help standardize the hydrodynamic forces in orbital shakers to increase the experimental accuracy and reproducibility and give students a better knowledge of the significance of the agitation process in microbial growth. |
Tipo: | Artigo |
Descrição: | The following material is available online at: https://journals.asm.org/doi/10.1128/jmbe.00099-23 |
URI: | https://hdl.handle.net/1822/88381 |
DOI: | 10.1128/jmbe.00099-23 |
ISSN: | 1935-7877 |
e-ISSN: | 1935-7885 |
Versão da editora: | https://journals.asm.org/doi/10.1128/jmbe.00099-23 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_56574_1.pdf | 165,43 kB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons