Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/88381

TitleA procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks
Author(s)Simões, Lúcia Chaves
Oliveira, Isabel
Borges, Anabela
Gomes, Inês Bezerra
Simões, Manuel
Keywordsagitation
hydrodynamic force
microbial growth
shake flasks
standardization
Issue dateDec-2023
PublisherAmerican Society for Microbiology
JournalJournal of Microbiology & Biology Education
CitationSimões, L. C., Oliveira, I., Borges, A., Gomes, I. B., & Simões, M. (2023, December 14). A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks. (L. J. MacDonald, Ed.), Journal of Microbiology & Biology Education. American Society for Microbiology. http://doi.org/10.1128/jmbe.00099-23
Abstract(s)Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size. Liquid agitation is implemented inherently to increase aeration, substrate transfer to the cells, and prevent sedimentation, disregarding the role of hydrodynamics in microbial growth and metabolism. Here, we present a simple approach to help standardize the hydrodynamic forces in orbital shakers to increase the experimental accuracy and reproducibility and give students a better knowledge of the significance of the agitation process in microbial growth.
TypeArticle
DescriptionThe following material is available online at: https://journals.asm.org/doi/10.1128/jmbe.00099-23
URIhttps://hdl.handle.net/1822/88381
DOI10.1128/jmbe.00099-23
ISSN1935-7877
e-ISSN1935-7885
Publisher versionhttps://journals.asm.org/doi/10.1128/jmbe.00099-23
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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