Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

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dc.contributor.authorGomez-Varela, Ana I.por
dc.contributor.authorStamov, Dimitar R.por
dc.contributor.authorMiranda, Adelaidepor
dc.contributor.authorAlves, Rosanapor
dc.contributor.authorBarata-Antunes, Claudiapor
dc.contributor.authorDambournet, Daphnepor
dc.contributor.authorDrubin, David G.por
dc.contributor.authorPaiva, Sandrapor
dc.contributor.authorDe Beule, Pieter A. A.por
dc.date.accessioned2021-05-27T09:56:03Z
dc.date.available2021-05-27T09:56:03Z
dc.date.issued2020
dc.date.updated2021-05-23T23:46:45Z
dc.description.abstractCorrelating data from different microscopy techniques holds the potential to discover new facets of signaling events in cellular biology. Here we report for the first time a hardware set-up capable of achieving simultaneous co-localized imaging of spatially correlated far-field super-resolution fluorescence microscopy and atomic force microscopy, a feat only obtained until now by fluorescence microscopy set-ups with spatial resolution restricted by the Abbe diffraction limit. We detail system integration and demonstrate system performance using sub-resolution fluorescent beads and applied to a test sample consisting of human bone osteosarcoma epithelial cells, with plasma membrane transporter 1 (MCT1) tagged with an enhanced green fluorescent protein (EGFP) at the N-terminal.por
dc.description.sponsorshipAna I. Gomez Varela wishes to acknowledge support from the Xunta de Galicia, Conselleria de Cultura, Educacion e Ordenacion Universitaria e da Conselleria de Economia, Emprego e Industria (Programa de axudas de apoio a etapa de formacion posdoutoral 2017). Adelaide Miranda and Pieter De Beule acknowledge financial support from Norte's Regional Operational Programme 2014-2020-Norte2020 (NORTE-01-0145-FEDER-000019). Sandra Paiva and Rosana Alves thank Fulbright Commission Portugal and Luso-American Development Foundation (FLAD) for their financial support to perform research work at UC Berkeley, California, USA. We thank the U.S. Embassy in Portugal for supporting David Drubin's visit to Portugal. We thank Ann Fisher of the UC Berkeley Cell Culture Facility for help with cell culture. We thank Dr. Kartoosh Heydari of the Cancer Research Lab Flow Cytometry Core Facility of UC Berkeley. Rosana Alves and Claudia Barata-Antunes are recipients of PhD fellowships from the Portuguese Foundation for Science and Technology (PD/BD/113813/2015 and PD/BD/135208/2017, respectively). The authors want to thank Nikon and Izasa Scientific for their support to the experiment by providing a N SIM-E microscope set-up on loan. We also gratefully acknowledge Dr. Kees van der Oord from Nikon Instruments Europe B.V. for his assistance with the SIM microscope as well as Paulo Madureira and Carlos Pitaes from IZASA Portugal and Jordi Recasens from IZASA Spain for their assistance with the integration of the SIM and AFM microscopes. Finally, we want to thank Benjamin Holmes for fruitful discussions and relentless support.por
dc.distributioninternationalpor
dc.identifier.citationGómez-Varela, A.I., Stamov, D.R., Miranda, A. et al. Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences. Sci Rep 10, 1122 (2020). https://doi.org/10.1038/s41598-020-57885-zpor
dc.identifier.doi10.1038/s41598-020-57885-zpor
dc.identifier.issn2045-2322
dc.identifier.pmid31980680
dc.identifier.urihttps://hdl.handle.net/1822/72887
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherNature Publishing Grouppor
dc.relationPD/BD/135208/2017
dc.relationFunction and regulation of Candida albicans and C. glabata carboxylic acid transporters and thei role in biofilm formation, antifungal drug resistance and phagocytosis [PD/BD/113813/2015]
dc.relationPD/BD/135208/2017por
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-020-57885-zpor
dc.rightsopenAccesspor
dc.subject.fosCiências Naturais::Ciências Biológicaspor
dc.subject.wosScience & Technology
dc.titleSimultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciencespor
dc.typearticlepor
dspace.entity.typePublicationen
oaire.awardNumberPD/BD/135208/2017
oaire.awardNumberPD/BD/113813/2015
oaire.awardTitlePD/BD/135208/2017
oaire.awardTitleFunction and regulation of Candida albicans and C. glabata carboxylic acid transporters and thei role in biofilm formation, antifungal drug resistance and phagocytosis [PD/BD/113813/2015]
oaire.awardURIhttps://hdl.handle.net/1822/100688
oaire.awardURIhttps://hdl.handle.net/1822/103326
oaire.citationIssue1por
oaire.citationVolume10por
oaire.funderIdentifierhttp://doi.org/10.13039/501100001871
oaire.funderIdentifierhttp://doi.org/10.13039/501100001871
oaire.funderNameFundação para a Ciência e a Tecnologia, I.P.
oaire.funderNameFundação para a Ciência e a Tecnologia, I.P.
relation.isProjectOfPublication39beab0f-a74e-4e04-ae2b-2c34451f7421
relation.isProjectOfPublicationf0ce0ff1-75c0-46f9-83df-bc0b1587c7d9
relation.isProjectOfPublication.latestForDiscovery39beab0f-a74e-4e04-ae2b-2c34451f7421
sdum.export.identifier10800
sdum.journalScientific Reportspor

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