Cytocompatible and biodegradable poly(D,L-lactide-coglycolide)/reduced graphene oxide scaffolds

Abstract

Applications of graphene in regenerative medicine have attracted the increasing attention of numerous research groups due to the specific properties that confers on biomaterials. In this paper, the degradation behavior of poly(d,l-lactide-co-glycolide (PLGA)/reduced graphene oxide (rGO) scaffolds obtained by thermally induced phase separation (TIPS) and lyophilization was studied in phosphate buffered saline (PBS) solution, at 37 °C during eight weeks. Additionally, the cytotoxicity of the different samples through the metabolic activity of L929 fibroblast cells was also addressed. Scanning electron microscopy tests show that the addition of rGO particles increases the pore size from 60 to 100 µm as well as their morphological definition. Scaffolds with 0.6 and 1% rGO concentrations lost more mass than those with lower filler content, that is, they degraded more quickly. The results obtained by differential scanning calorimetry indicate that the rGO particles restrict the movement of the macromolecular chain segments due to the formation of hydrogen bonds and electrostatic attraction. The electrical conductivity tests show that the addition of rGO leads to a rapid transition from insulating to conductive scaffolds with a percolation value of ≈ 0.5 w/w. All the different PLGA samples with different rGO content up to 1% present no cytotoxic behaviour for L929 fibroblast cells, being therefor suitable for biomedical applications.