Human cells response to Non- Candida albicans Candida infection

Abstract

This study evaluated the influence of adhered Candida spp (C.tropicalis ATCC 750, C. parapsilosis ATCC 2201, C. glabrata ATCC 2001 and C. albicans SC5413) on the biological behavior of human cells (HeLa and TCC-SUP). A yeast suspension was added to a confluent layer of human cells and after 2 and 24h of incubation yeast cells were detached for CFU counts. All Candida spp assayed adhered to epithelial cells, although C. glabrata was the species adhering in higher extent to both epithelial cell lines. From 2 to 24h there is a significant increase in the number of C. glabrata adhered to TCC-SUP cells, while no increase in the number of C. glabrata colonizing HeLa cells was observed. An opposite result was observed for C. tropicalis; there was no increase in the number of adherent cells to TCC-SUP cells, but there was an increase for HeLa cells. The metabolic activity of HeLa cells decreased after a period of 24h of Candida infection, especially in the presence C. tropicalis. The biological response of TCC-SUP cells was different from the one observed with HeLa cells, the presence of C. albicans and C. parapsilosis induced an increase in the metabolic activity of TCC-SUP cells, while C. tropicalis and C. glabrata promoted a decrease.. After 24h of C. albicans adhesion, the highest human cells damage was detected in HeLa cells (measured by LDH release), while C.tropicalis seems to induce higher LDH release after 24h in contact with TCC-SUP cells. The biological response of human cells showed to be dependent on their origin and on the colonizing yeast species/strain, and no direct relation between the number of adherent cells and the biological response was observed.