An efficient antioxidant system and heavy metal exclusion from leaves make Solanum cheesmaniae more tolerant to Cu than its cultivated counterpart

Abstract

Copper (Cu) is an abundant metal in the environment coming from anthropogenic activities and natural sources that, in excess, easily becomes phytotoxic to most species, being its accumulation in plants considered an environmental threat. This study aimed to compare the physiological and molecular responses of Solanum lycopersicum and its wild counterpart Solanum cheesmaniae to Cu stress. In particular, we wanted to address the hypothesis that S. cheesmaniae is more adapted to Cu stress than S. lycopersicum, since the former is equipped with a more efficient antioxidant defense system than the latter. Biomarkers of oxidative status (lipid peroxidation, hydrogen peroxide (H2O2) and superoxide anion (O.− 2 ) levels) revealed a more pronounced imbalance in the redox homeostasis in shoots of S. lycopersicum than in S. cheesmaniae in response to Cu. Furthermore, the activity of key antioxidant enzymes clearly differed in both species in response to Cu. Catalase (CAT) activity increased in S. cheesmaniae shoots but decreased in the domestic species, as well as ascorbate peroxidase (APX). Both species preferentially accumulated Cu in the radicular system, although a great increase in the aerial parts of S. lycopersicum was measured, while in leaves of Cu- treated S. cheesmaniae, the levels of Cu were not changed. Overall, results validated the hypothesis that S. cheesmaniae is more tolerant to excess Cu than S. lycopersicum and the data provided will help the development of breeding strategies toward the improvement of the resistance/tolerance of cultivated tomato species to heavy metal stress.