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https://hdl.handle.net/1822/10495
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Campo DC | Valor | Idioma |
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dc.contributor.author | Franklin, Gregory | - |
dc.contributor.author | Conceição, Luís F. R. | - |
dc.contributor.author | Kombrink, E. | - |
dc.contributor.author | Dias, Alberto Carlos Pires | - |
dc.date.accessioned | 2010-03-18T16:36:27Z | - |
dc.date.available | 2010-03-18T16:36:27Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | "Planta". ISSN 0032-0935. 227:6 (2008) 1401–1408. | por |
dc.identifier.issn | 0032-0935 | por |
dc.identifier.issn | 1432-2048 | por |
dc.identifier.uri | https://hdl.handle.net/1822/10495 | - |
dc.description.abstract | Plant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the Wrst evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation. | por |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) - POCTI/AGR/40283/2001, SFRH/BPD/17102/ 2004 | por |
dc.language.iso | eng | por |
dc.publisher | Springer Verlag | por |
dc.rights | openAccess | por |
dc.subject | Agrobacterium | por |
dc.subject | Hypericum | por |
dc.subject | Plant defense response | por |
dc.subject | Plant recalcitrance | por |
dc.title | Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | The original publication is available at www.springerlink.com | por |
sdum.number | 6 | por |
sdum.pagination | 1401–1408 | por |
sdum.publicationstatus | published | por |
sdum.volume | 227 | por |
oaire.citationStartPage | 1401 | por |
oaire.citationEndPage | 1408 | por |
oaire.citationIssue | 6 | por |
oaire.citationVolume | 227 | por |
dc.identifier.doi | 10.1007/s00425-008-0691-7 | por |
dc.identifier.pmid | 18247048 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Planta | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Franklin et al 2008 Planta.pdf | Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation | 666,53 kB | Adobe PDF | Ver/Abrir |