Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/10495
Título: | Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation |
Autor(es): | Franklin, Gregory Conceição, Luís F. R. Kombrink, E. Dias, Alberto Carlos Pires |
Palavras-chave: | Agrobacterium Hypericum Plant defense response Plant recalcitrance |
Data: | 2008 |
Editora: | Springer Verlag |
Revista: | Planta |
Citação: | "Planta". ISSN 0032-0935. 227:6 (2008) 1401–1408. |
Resumo(s): | Plant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the Wrst evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/10495 |
DOI: | 10.1007/s00425-008-0691-7 |
ISSN: | 0032-0935 1432-2048 |
Versão da editora: | The original publication is available at www.springerlink.com |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Franklin et al 2008 Planta.pdf | Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation | 666,53 kB | Adobe PDF | Ver/Abrir |